BACKGROUND: The transmissible degenerative encephalopathies (TDEs) constitute a distinct group of diseases (scrapie in sheep, bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease (CJD) in humans). The causal agents are not fully characterized, but are known to be resistant to most inactivation procedures. Ruminants appear to be particularly susceptible to TDEs. The concentrations of hydrochloric acid in their digestive tracts are significantly lower than in monogastric species. METHODS: The aim of the study was to examine the role of gastric acidity in the protection of mice against infection after intragastric administration of different doses of a scrapie agent. Gastric acidity levels in mice were reduced by adding ranitidine to the drinking water and the animals were observed for neurological symptoms and at sacrifice examined microscopically for spongiform lesions in the brain. RESULTS: The lower doses of infectious material induced disease significantly more often in mice given ranitidine compared with the controls. CONCLUSION: These data indicate that the normal levels of gastric acidity in mice protect them to some extent from infection with low doses of scrapie agent. This finding is potentially relevant to the pathogenesis of the variant form of CJD, which appears to be associated with the consumption of BSE-infected food products.
BACKGROUND: The transmissible degenerative encephalopathies (TDEs) constitute a distinct group of diseases (scrapie in sheep, bovine spongiform encephalopathy (BSE) and Creutzfeldt-Jakob disease (CJD) in humans). The causal agents are not fully characterized, but are known to be resistant to most inactivation procedures. Ruminants appear to be particularly susceptible to TDEs. The concentrations of hydrochloric acid in their digestive tracts are significantly lower than in monogastric species. METHODS: The aim of the study was to examine the role of gastric acidity in the protection of mice against infection after intragastric administration of different doses of a scrapie agent. Gastric acidity levels in mice were reduced by adding ranitidine to the drinking water and the animals were observed for neurological symptoms and at sacrifice examined microscopically for spongiform lesions in the brain. RESULTS: The lower doses of infectious material induced disease significantly more often in mice given ranitidine compared with the controls. CONCLUSION: These data indicate that the normal levels of gastric acidity in mice protect them to some extent from infection with low doses of scrapie agent. This finding is potentially relevant to the pathogenesis of the variant form of CJD, which appears to be associated with the consumption of BSE-infected food products.
Authors: Christopher J Johnson; Kristen E Phillips; Peter T Schramm; Debbie McKenzie; Judd M Aiken; Joel A Pedersen Journal: PLoS Pathog Date: 2006-04-14 Impact factor: 6.823
Authors: Christopher M Vickery; Katy E Beck; Marion M Simmons; Stephen A C Hawkins; John Spiropoulos Journal: Int J Exp Pathol Date: 2013-10 Impact factor: 1.925
Authors: Christopher J Johnson; Joel A Pedersen; Rick J Chappell; Debbie McKenzie; Judd M Aiken Journal: PLoS Pathog Date: 2007-07 Impact factor: 6.823