Influence of An Adenosine Receptor Antagonist on Neurotransmitters in Parkinsonian Mice.

The effects of the adenosine A(2a) receptor antagonist, Quinazoline, on the monoamine transmitters NA, DA, 5-HT and 5-HIAA was studied using fluorecent method, and the influence of MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and Quinazoline (CP66713) on the GABA positive reaction cells in globus pallidus was determined by immunohistochemistry technique in MPTP treated Parkinsonian mice. The results showed that MPTP decreased the content of DA, 5-HIAA and increased 5-HT, GABA obviously(pp<0.01). Quinazoline functioned opposite the action of MPTP, increasing the content of DA, 5-HIAA and decreasing the 5-HT. Quinazoline also reduced the GABA-positive reaction cells in globus pallidus of control mice and maintained the level of GABA-positive reaction cells of globus pallidus in MPTP treated mice, making it similar with the level of control.