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Literature DB >> 12057972

PII T-loop mutations affecting signal transduction to NtrB also abolish yeast two-hybrid interactions.

Isabel Martínez-Argudo¹, Asunción Contreras.

Abstract

Mutations A49P and Delta47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997). We show here that these mutations also impair interactions between PII and NtrB in the yeast two-hybrid system, indicating that defects in NtrB regulation closely reflect binding impairment. The reported results underline the strength of two-hybrid assays for analysis of interactions involving the T loop of PII proteins.

Entities: Gene Mutation Species

Mesh：

Substances：

Year: 2002 PMID： 12057972 PMCID： PMC135122 DOI： 10.1128/JB.184.13.3746-3748.2002

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

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