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Literature DB >> 12054925

Analyzing mitochondrial changes during apoptosis.

Abstract

Mitochondria play a central role in programmed cell death through the release of cytochrome c and other proapoptotic factors. Fluorescence microscopy is used to visualize cytochrome c translocation and loss of mitochondrial membrane potential. Flow cytometry can also be used to measure mitochondrial membrane potential. Cytochrome c content in cytosol and mitochondria can be determined by immunoblotting after subcellular fractionation or selective permeabilization with digitonin. Isolated mitochondria can be used to study the mechanism of cytochrome c release. This article summarizes some of the more widely used methods to assess mitochondrial alterations in apoptosis.

Entities: Chemical Gene

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Year: 2002 PMID： 12054925 DOI： 10.1016/S1046-2023(02)00040-3

Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608

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Cited

22 in total

1. Human mitochondrial DNA with large deletions repopulates organelles faster than full-length genomes under relaxed copy number control.

Authors: Francisca Diaz; Maria Pilar Bayona-Bafaluy; Michele Rana; Marialejandra Mora; Huiling Hao; Carlos T Moraes
Journal: Nucleic Acids Res Date: 2002-11-01 Impact factor: 16.971

2. A high-throughput 3-parameter flow cytometry-based cell death assay.

Authors: Eric J Buenz; Paul J Limburg; Charles L Howe
Journal: Cytometry A Date: 2007-03 Impact factor: 4.355

3. Establishment and characterization of Prnp knockdown neuroblastoma cells using dual microRNA-mediated RNA interference.

Authors: Sang-Gyun Kang; Yu-Mi Roh; Agnes Lau; David Westaway; Debbie McKenzie; Judd Aiken; Yong-Sun Kim; Han Sang Yoo
Journal: Prion Date: 2011-04-01 Impact factor: 3.931

4. Plasma membrane localization and function of the estrogen receptor alpha variant (ER46) in human endothelial cells.

Authors: Lei Li; M Page Haynes; Jeffrey R Bender
Journal: Proc Natl Acad Sci U S A Date: 2003-04-07 Impact factor: 11.205

5. Control of insulin secretion by cytochrome C and calcium signaling in islets with impaired metabolism.

Authors: Austin M Rountree; Adam S Neal; Mark Lisowski; Norma Rizzo; Jared Radtke; Sarah White; Dan S Luciani; Francis Kim; Christiane S Hampe; Ian R Sweet
Journal: J Biol Chem Date: 2014-05-19 Impact factor: 5.157

6. Selective induction of tumor cell apoptosis by a novel P450-mediated reactive oxygen species (ROS) inducer methyl 3-(4-nitrophenyl) propiolate.

Authors: Xiaoxiao Sun; Midan Ai; Ying Wang; Shensi Shen; Yuan Gu; Yi Jin; Zuyu Zhou; Yaqiu Long; Qiang Yu
Journal: J Biol Chem Date: 2013-02-04 Impact factor: 5.157

Analyzing mitochondrial changes during apoptosis.

1. Human mitochondrial DNA with large deletions repopulates organelles faster than full-length genomes under relaxed copy number control.

2. A high-throughput 3-parameter flow cytometry-based cell death assay.

3. Establishment and characterization of Prnp knockdown neuroblastoma cells using dual microRNA-mediated RNA interference.

4. Plasma membrane localization and function of the estrogen receptor alpha variant (ER46) in human endothelial cells.

5. Control of insulin secretion by cytochrome C and calcium signaling in islets with impaired metabolism.

6. Selective induction of tumor cell apoptosis by a novel P450-mediated reactive oxygen species (ROS) inducer methyl 3-(4-nitrophenyl) propiolate.

7. Mitochondrial survivin inhibits apoptosis and promotes tumorigenesis.

8. Mitochondrial damage as death inducer in heart-derived H9c2 cells: more than one way for an early demise.

9. miR-217-5p induces apoptosis by directly targeting PRKCI, BAG3, ITGAV and MAPK1 in colorectal cancer cells.

10. Promotion of Caspase Activation by Caspase-9-mediated Feedback Amplification of Mitochondrial Damage.