Barotolerant E.coli Induced by High Hydrostatic Pressure.

The barotolerant E.coli TG1P, DH5alphaP, HB101P were obtained from TG1,DH5alpha, HB101 after treatment with high hydrostatic pressure, and the survival rates of the formers raised 2--3 magnitude, from 1.06x10(-6), 2.98x10(-4), 3.65x10(-3) to 8.31x10(-3), 3.40x10(-1), 1.69x10(-1),respectively. Comparison of partial proteomes of wild and barotolerant bacteria of TG1 and DH5alpha with two-dimensional electrophoresis showed that three proteins were obviously highly expressed in barotolerant bacteria. The N terminal sequence and molecular weight of one protein was shown to be V(L)EAGEFFMRA and 21 kD, with high identity to a known outmembrane protein in E.coli.