| Literature DB >> 12052710 |
Olaf Kinstler1, Graham Molineux, Michael Treuheit, David Ladd, Colin Gegg.
Abstract
A site-directed method of joining proteins to poly(ethylene glycol) is presented which allows for the preparation of essentially homogeneous PEG-protein derivatives with a single PEG chain conjugated to the amine terminus of the protein. This selectivity is achieved by conducting the reductive alkylation of proteins with PEG-aldehydes at lower pH. Working examples demonstrating the application of this method to improve the delivery characteristics and therapeutic value of several proteins are provided.Entities:
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Year: 2002 PMID： 12052710 DOI： 10.1016/s0169-409x(02)00023-6
Source DB: PubMed Journal: Adv Drug Deliv Rev ISSN： 0169-409X Impact factor: 15.470