| Literature DB >> 12052355 |
Rahul Singhal1, Anamika Gambhir, M K Pandey, S Annapoorni, B D Malhotra.
Abstract
Urease was immobilized in mixed monolayers of poly(N-vinyl carbazole) (PNVK) and stearic acid (SA) formed at an air-water interface. The monolayers were transferred onto indium-tin-oxide (ITO) coated glass plates using Langmuir-Blodgett (LB) film deposition technique. Urease immobilized on PNVK/SA LB films, characterized using FTIR and UV-visible spectroscopy, was found to exhibit increased stability over a wide pH (6.5-8.5) and temperature (25-50 degrees C) range. Potentiometric measurements on these urease electrodes were carried out using an ammonium ion analyzer. Two values for K(m)(app) were obtained at lower and higher concentrations of substrate urea.Entities:
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Year: 2002 PMID： 12052355 DOI： 10.1016/s0956-5663(02)00020-9
Source DB: PubMed Journal: Biosens Bioelectron ISSN： 0956-5663 Impact factor: 10.618