Literature DB >> 12050777

Vitrification of human spermatozoa without cryoprotectants.

F Nawroth1, V Isachenko, S Dessole, G Rahimi, M Farina, N Vargiu, P Mallmann, M Dattena, G Capobianco, D Peters, I Orth, E Isachenko.   

Abstract

The aim of our study was to investigate the effects of vitrification (cooling rate approximately 10000(C/min) without cryoprotectants on swim-up prepared human spermatozoa in comparison to standard conventional freezing with cryoprotectants. Motility, morphology, rate of viability and acrosome reaction of spermatozoa were evaluated. The described method of cryopreservation of human spermatozoa by direct plunging into liquid nitrogen slush without cryoprotectants was effective and could be recommended for routine IVF.

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Year:  2002        PMID： 12050777

Source DB:  PubMed          Journal:  Cryo Letters        ISSN： 0143-2044            Impact factor:   1.066


  19 in total

1.  Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa.

Authors:  Sinan Ozkavukcu; Esra Erdemli; Ayca Isik; Derya Oztuna; Sercin Karahuseyinoglu
Journal:  J Assist Reprod Genet       Date:  2008-08-13       Impact factor: 3.412

2.  Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.

Authors:  Rafael Cuevas-Uribe; Huiping Yang; Jonathan Daly; Markita G Savage; Ronald B Walter; Terrence R Tiersch
Journal:  Zebrafish       Date:  2011-09-01       Impact factor: 1.985

3.  Principles of Ice-Free Cryopreservation by Vitrification.

Authors:  Gregory M Fahy; Brian Wowk
Journal:  Methods Mol Biol       Date:  2021

Review 4.  ART strategies in Klinefelter syndrome.

Authors:  Wei Chen; Ming Zhu Bai; Yixia Yang; Di Sun; Sufang Wu; Jian Sun; Yu Wu; Youji Feng; Youheng Wei; Zijiang Chen; Zhenbo Zhang
Journal:  J Assist Reprod Genet       Date:  2020-06-20       Impact factor: 3.412

5.  Vitrification of Sperm from Marine Fishes: Effect on Motility and Membrane Integrity.

Authors:  Rafael Cuevas-Uribe; Edward J Chesney; Jonathan Daly; Terrence R Tiersch
Journal:  Aquac Res       Date:  2015-06-01       Impact factor: 2.082

6.  Cryopreservation of very low numbers of spermatozoa from male patients undergoing infertility treatment using agarose capsules.

Authors:  Shota Hatakeyama; Susumu Tokuoka; Hiroyuki Abe; Yasuyuki Araki; Yasuhisa Araki
Journal:  Hum Cell       Date:  2017-03-01       Impact factor: 4.174

7.  Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant.

Authors:  Qiaoxiang Dong; Liane M Correa; Catherine A VandeVoort
Journal:  Cryobiology       Date:  2008-10-21       Impact factor: 2.487

8.  Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent.

Authors:  Mengying Wang; Evgenia Isachenko; Gohar Rahimi; Peter Mallmann; Vladimir Isachenko
Journal:  Methods Mol Biol       Date:  2021

9.  Human Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART.

Authors:  Marlea Di Santo; Nicoletta Tarozzi; Marco Nadalini; Andrea Borini
Journal:  Adv Urol       Date:  2011-12-13

10.  Unraveling Subcellular and Ultrastructural Changes During Vitrification of Human Spermatozoa: Effect of a Mitochondria-Targeted Antioxidant and a Permeable Cryoprotectant.

Authors:  Pradeep Kumar; Mengying Wang; Evgenia Isachenko; Gohar Rahimi; Peter Mallmann; Wanxue Wang; Melanie von Brandenstein; Vladimir Isachenko
Journal:  Front Cell Dev Biol       Date:  2021-07-02
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