Literature DB >> 1204808

A method for distinction between RNA and DNA in aldehyde and osmiumtetroxide-fixed electron microscopic autoradiographs.

D Egger, K Bienz.   

Abstract

A simple method for distinction between RNA- and DNA-containing structures in aldehyde- and osmiumtextroxide-fixed electron microscopic autoradiographs (or ordinary thin sections) is described: the developer and the acetic acid used for processing autoradiographs extract selectively uranium acetate from DNA containing-structures which, after staining with lead citrate, leads to a characteristically 'bleached' appearance of the DNA.

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Year:  1975        PMID： 1204808     DOI： 10.1007/bf01945835

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  6 in total

1.  Light and electron microscopic autoradiography of coxsackievirus A 1 infected muscles: viral RNA synthesis and inhibition of host cell RNA synthesis.

Authors:  K Bienz; D Egger; G Bienz-Isler; H Loeffler
Journal:  Arch Gesamte Virusforsch       Date:  1972

2.  Critical evaluation of specificity in electron microscopical radioautography in animal tissues.

Authors:  A Monneron; Y Moulé
Journal:  Exp Cell Res       Date:  1969-08       Impact factor: 3.905

3.  A new staining procedure for electron microscopical cytology.

Authors:  W Bernhard
Journal:  J Ultrastruct Res       Date:  1969-05

4.  High-resolution autoradiography. I. Methods.

Authors:  L G CARO; R P VAN TUBERGEN; J A KOLB
Journal:  J Cell Biol       Date:  1962-11       Impact factor: 10.539

5.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539

6.  Improvements in epoxy resin embedding methods.

Authors:  J H LUFT
Journal:  J Biophys Biochem Cytol       Date:  1961-02
  6 in total

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