| Literature DB >> 12047358 |
A F da Mota1, J E Gabriel, M L Martinez, L L Coutinho.
Abstract
Brazilian dairy Gir (Bos indicus) cattle are a tropical, well-adapted breed, for which no information on the major histocompatibility complex (MHC) is presently available. The second exon of the bovine lymphocyte antigen (BoLA-DRB3) was amplified by polymerase chain reaction (PCR) of DNA samples from 28 Brazilian dairy Gir cows. Two experimental genotyping approaches were used: direct sequencing of PCR gene products (PCR-DS) and sequencing of cloned PCR fragments (S-CLO). Results demonstrate the viability of both typing approaches. PCR-DS allowed typing of 39% of the animals while the remainder were genotyped by S-CLO. Seventeen BoLA-DRB3 alleles were assigned, including some that were only recently described for zebu cattle. Allelic frequencies ranged from 0.02 to 0.18. The most frequent alleles were *3601 (frequency = 0.18), *2201 (0.14) and *2101 (0.11).Entities:
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Year: 2002 PMID： 12047358 DOI： 10.1046/j.1365-2370.2002.00294.x
Source DB: PubMed Journal: Eur J Immunogenet ISSN： 0960-7420