| Literature DB >> 12047229 |
N Navani1, P K Jain, S Gupta, B S Sisodia, S Kumar.
Abstract
One hundred and eight microsatellite primer pairs, originally identified from cattle, were evaluated for their applicability in buffalo. Eighty-one primer pairs (75%) amplified discrete products, and of these, 61 pairs (56%) gave polymorphic band patterns on a panel of 25 buffaloes. The mean number of alleles per polymorphic marker was 4.50 +/- 0.20, and the mean heterozygosity per polymorphic marker was 0.66 +/- 0.02. Successful genotyping of buffaloes using cattle specific primers suggests that the latter can be a valuable resource for genome analysis in bubaline species.Entities:
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Year: 2002 PMID: 12047229 DOI: 10.1046/j.1365-2052.2002.00823.x
Source DB: PubMed Journal: Anim Genet ISSN: 0268-9146 Impact factor: 3.169