Literature DB >> 12038566

Characterization of a highly thermostable extracellular lipase from Lactobacillus plantarum.

Maria de Fátima Silva Lopes1, Ana Lúcia Leitão, Manuela Regalla, J J Figueiredo Marques, Manuel José Teixeira Carrondo, Maria Teresa Barreto Crespo.   

Abstract

After screening for the presence of lipase activity in lactobacilli isolated from "chouriço", a traditional Portuguese dry fermented sausage, a strain of Lactobacillus plantarum (DSMZ 12028) was chosen for extracellular lipase characterisation and purification. Proteinase K did not significantly affect lipolytic activity, as opposed to trypsin, which completely eliminated this activity. Among NaCl, Ca2+, EDTA, BSA, glycerol, Mn2+ and Mg2+, only Mn2+ and Mg2+ stimulated the lipase. Purification by gel filtration chromatography and gel electrophoresis revealed four bands, between 98 and 45 kDa, all with lipolytic activity against olive oil.

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Year:  2002        PMID： 12038566     DOI： 10.1016/s0168-1605(02)00013-2

Source DB:  PubMed          Journal:  Int J Food Microbiol        ISSN： 0168-1605            Impact factor:   5.277


  9 in total

Review 1.  Lactic acid bacteria as a potential source of enzymes for use in vinification.

Authors:  Angela Matthews; Antonio Grimaldi; Michelle Walker; Eveline Bartowsky; Paul Grbin; Vladimir Jiranek
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

2.  Overexpression, purification, and biochemical characterization of the esterase Est0796 from Lactobacillus plantarum WCFS1.

Authors:  Inmaculada Navarro-González; Navarro-González Inmaculada; Sánchez-Ferrer Alvaro; García-Carmona Francisco
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695

3.  Molecular cloning and characterization of a thermostable lipase from deep-sea thermophile Geobacillus sp. EPT9.

Authors:  Yanbing Zhu; Hebin Li; Hui Ni; Anfeng Xiao; Lijun Li; Huinong Cai
Journal:  World J Microbiol Biotechnol       Date:  2014-11-12       Impact factor: 3.312

4.  Cloning, expression, purification, and characterization of a novel esterase from Lactobacillus plantarum.

Authors:  Fábio Cristiano Angonesi Brod; Javier Vernal; Jean Borges Bertoldo; Hernán Terenzi; Ana Carolina Maisonnave Arisi
Journal:  Mol Biotechnol       Date:  2010-03       Impact factor: 2.695

Review 5.  Production strategies and biotechnological relevance of microbial lipases: a review.

Authors:  Adegoke Isiaka Adetunji; Ademola Olufolahan Olaniran
Journal:  Braz J Microbiol       Date:  2021-04-27       Impact factor: 2.476

6.  Lipolytic potential of Aspergillus japonicus LAB01: production, partial purification, and characterisation of an extracellular lipase.

Authors:  Lívia Tereza Andrade Souza; Jamil S Oliveira; Vera L dos Santos; Wiliam C B Regis; Marcelo M Santoro; Rodrigo R Resende
Journal:  Biomed Res Int       Date:  2014-10-29       Impact factor: 3.411

7.  Partial purification, characterization and immobilization of a novel lipase from a native isolate of Lactobacillus fermentum.

Authors:  Foruzan Fathi; Rouha Kasra-Kermanshahi; Zahra Moosavi-Nejad; Elahe Mobarak Qamsari
Journal:  Iran J Microbiol       Date:  2021-12

8.  Thermostable lipases from the extreme thermophilic anaerobic bacteria Thermoanaerobacter thermohydrosulfuricus SOL1 and Caldanaerobacter subterraneus subsp. tengcongensis.

Authors:  Marina Royter; M Schmidt; C Elend; H Höbenreich; T Schäfer; U T Bornscheuer; G Antranikian
Journal:  Extremophiles       Date:  2009-07-05       Impact factor: 2.395

9.  Purification and Characterization of Lipase Produced by Leuconostoc mesenteroides Subsp. mesenteroides ATCC 8293 Using an Aqueous Two-Phase System (ATPS) Composed of Triton X-100 and Maltitol.

Authors:  Nurfadhilah Hidayah Eko Sukohidayat; Mohammad Zarei; Badlishah Sham Baharin; Mohd Yazid Manap
Journal:  Molecules       Date:  2018-07-20       Impact factor: 4.411
  9 in total

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