Literature DB >> 12037311

Expression, purification and crystallization of a collagen-binding fragment of Yersinia adhesin YadA.

Heli Nummelin1, Yasmin El Tahir, Pauli Ollikka, Mikael Skurnik, Adrian Goldman.   

Abstract

A recombinant form of a collagen-binding fragment of Yersinia enterocolitica serotype O:3 adhesin YadA with an N-terminal polyhistidine affinity tag has been produced in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion technique. Crystals belong to the trigonal space group R3, with unit-cell parameters a = b = 67.05, c = 221.95 A, and diffract to 1.55 A resolution on a synchrotron-radiation source.

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Year:  2002        PMID： 12037311     DOI： 10.1107/s0907444902005231

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  6 in total

1.  yadBC of Yersinia pestis, a new virulence determinant for bubonic plague.

Authors:  Stanislav Forman; Christine R Wulff; Tanya Myers-Morales; Clarissa Cowan; Robert D Perry; Susan C Straley
Journal:  Infect Immun       Date:  2007-11-19       Impact factor: 3.441

2.  First analysis of a bacterial collagen-binding protein with collagen Toolkits: promiscuous binding of YadA to collagens may explain how YadA interferes with host processes.

Authors:  Jack C Leo; Heli Elovaara; Dominique Bihan; Nicholas Pugh; Sami K Kilpinen; Nicolas Raynal; Mikael Skurnik; Richard W Farndale; Adrian Goldman
Journal:  Infect Immun       Date:  2010-05-03       Impact factor: 3.441

3.  Structural Basis for Toughness and Flexibility in the C-terminal Passenger Domain of an Acinetobacter Trimeric Autotransporter Adhesin.

Authors:  Kotaro Koiwai; Marcus D Hartmann; Dirk Linke; Andrei N Lupas; Katsutoshi Hori
Journal:  J Biol Chem       Date:  2015-12-23       Impact factor: 5.157

4.  The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll.

Authors:  Heli Nummelin; Michael C Merckel; Jack C Leo; Hilkka Lankinen; Mikael Skurnik; Adrian Goldman
Journal:  EMBO J       Date:  2004-02-05       Impact factor: 11.598

5.  The Yersinia adhesin YadA binds to a collagenous triple-helical conformation but without sequence specificity.

Authors:  Jack C Leo; Heli Elovaara; Barbara Brodsky; Mikael Skurnik; Adrian Goldman
Journal:  Protein Eng Des Sel       Date:  2008-05-07       Impact factor: 1.650

6.  A novel intrinsically disordered outer membrane lipoprotein of Aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8.

Authors:  Tuuli Ahlstrand; Heidi Tuominen; Arzu Beklen; Annamari Torittu; Jan Oscarsson; Raija Sormunen; Marja T Pöllänen; Perttu Permi; Riikka Ihalin
Journal:  Virulence       Date:  2016-07-26       Impact factor: 5.882
  6 in total

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