Literature DB >> 12037103

Comparative study of two methods for RNA extraction prior to detection of resistance to human immunodeficiency virus type 1 with the line probe assay.

Jose María Eiros1, Cristina Labayru, Beatriz Hernández, Raúl Ortiz De Lejarazu, Antonio Rodríguez Torres.   

Abstract

We evaluated two methods from Roche and Promega for RNA extraction prior to the genotypic detection of human immunodeficiency virus type 1 resistance by line probe assay (LiPA). Fifty plasma RNA extracts were processed in parallel by LiPA. Results obtained by the Roche method were superior in the proportion of amplified samples, the percentage of mutated samples, and band intensity.

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Year:  2002        PMID： 12037103      PMCID： PMC130752          DOI： 10.1128/JCM.40.6.2257-2259.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Isolation of HIV-1 RNA from plasma: evaluation of eight different extraction methods.

Authors:  C Verhofstede; K Fransen; D Marissens; R Verhelst; G van der Groen; S Lauwers; G Zissis; J Plum
Journal:  J Virol Methods       Date:  1996-07       Impact factor: 2.014

2.  Isolation of HIV-1 RNA from plasma: evaluation of seven different methods for extraction (part two).

Authors:  K Fransen; D Mortier; L Heyndrickx; C Verhofstede; W Janssens; G van der Groen
Journal:  J Virol Methods       Date:  1998-12       Impact factor: 2.014

3.  Improved conditions for extraction and amplification of human immunodeficiency virus type 1 RNA from plasma samples with low viral load.

Authors:  M L Villahermosa; M Thomson; E Vázquez de Parga; M T Cuevas; G Contreras; L Pérez-Alvarez; E Delgado; N Manjón; L Medrano; R Nájera
Journal:  J Hum Virol       Date:  2000 Jan-Feb

4.  Evaluation of mixtures of wild-type HIV-1 and HIV-1 with resistance point mutations against reverse transcriptase inhibitors.

Authors:  M L Villahermosa; G Contreras; L Pérez-Alvarez; F Bru; L Medrano; E Delgado; C Colomo; M Thomson; R Nájera
Journal:  Antivir Ther       Date:  1998

5.  Highly sensitive methods for quantitation of human immunodeficiency virus type 1 RNA from plasma, cells, and tissues.

Authors:  M Fischer; W Huber; A Kallivroussis; P Ott; M Opravil; R Lüthy; R Weber; R W Cone
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

6.  Detection of low HIV-1 RNA levels in plasma.

Authors:  G A Schockmel; S Yerly; L Perrin
Journal:  J Acquir Immune Defic Syndr Hum Retrovirol       Date:  1997-02-01

7.  Antiretroviral drug resistance testing in adult HIV-1 infection: recommendations of an International AIDS Society-USA Panel.

Authors:  M S Hirsch; F Brun-Vézinet; R T D'Aquila; S M Hammer; V A Johnson; D R Kuritzkes; C Loveday; J W Mellors; B Clotet; B Conway; L M Demeter; S Vella; D M Jacobsen; D D Richman
Journal:  JAMA       Date:  2000-05-10       Impact factor: 56.272

8.  Comparison of QIAamp HCV kit spin columns, silica beads, and phenol-chloroform for recovering human immunodeficiency virus type 1 RNA from plasma.

Authors:  R W Shafer; D J Levee; M A Winters; K L Richmond; D Huang; T C Merigan
Journal:  J Clin Microbiol       Date:  1997-02       Impact factor: 5.948

9.  Line probe assay for rapid detection of drug-selected mutations in the human immunodeficiency virus type 1 reverse transcriptase gene.

Authors:  L Stuyver; A Wyseur; A Rombout; J Louwagie; T Scarcez; C Verhofstede; D Rimland; R F Schinazi; R Rossau
Journal:  Antimicrob Agents Chemother       Date:  1997-02       Impact factor: 5.191

10.  Recovery and analysis of human immunodeficiency virus type 1 (HIV) RNA sequences from plasma samples with low HIV RNA levels.

Authors:  J Niubò; W Li; K Henry; A Erice
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948
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