BACKGROUND: Although a few growth media are available for selective isolation of the cariogenic bacteria, Streptococcus mutans (S. mutans), it is still unclear as to which is the most efficacious. This study compared the selectivity and sensitivity of five different media for growing a laboratory strain of S. mutans (NCTC 10449), and for enumerating S. mutans from teeth of a group of young children, aged 2-10 years. METHODS: The media tested in this study were mitis salivarius with bacitracin (MSB), mitis salivariuskanamycin-bacitracin (MSKB), glucose-sucrose-tellurite-bacitracin (GSTB), trypticase soy-sucrose-bacitracin (TYS20B) and tryptone-yeast-cysteine-sucrose-bacitracin (TYCSB) agars. These were prepared according to the respective manufacturer's instructions. RESULTS: The results showed that at concentrations of bacteria 1 x 10(3) to 1 x 10(10)/mL, the recovery of the laboratory S. mutans strain was highest in TYCSB agar, followed by in descending order by TYS20B, MSB, GSTB, and MSKB (p<0.01). Similar results were obtained using dental plaque samples collected from swabs of the teeth of a group of children. In the clinical samples, TYCSB again showed the highest recovery rates of S. mutans compared to the other four media. Results were reproduced at dilutions of the samples at 1:20 x 10(6) to 1:2 x 10(6), and S. mutans concentrations of 1.6 to 7.7 Log 10 CFU/mL. In addition, there were highest ratios of mutans to non-mutans bacteria in TYCSB compared to the other media, suggesting high selectivity of this media for mutans species. CONCLUSION: The results of our study suggest that TYCSB is the most sensitive and selective media for culture of S. mutans for laboratory and clinical studies.
BACKGROUND: Although a few growth media are available for selective isolation of the cariogenic bacteria, Streptococcus mutans (S. mutans), it is still unclear as to which is the most efficacious. This study compared the selectivity and sensitivity of five different media for growing a laboratory strain of S. mutans (NCTC 10449), and for enumerating S. mutans from teeth of a group of young children, aged 2-10 years. METHODS: The media tested in this study were mitis salivarius with bacitracin (MSB), mitis salivariuskanamycin-bacitracin (MSKB), glucose-sucrose-tellurite-bacitracin (GSTB), trypticase soy-sucrose-bacitracin (TYS20B) and tryptone-yeast-cysteine-sucrose-bacitracin (TYCSB) agars. These were prepared according to the respective manufacturer's instructions. RESULTS: The results showed that at concentrations of bacteria 1 x 10(3) to 1 x 10(10)/mL, the recovery of the laboratory S. mutans strain was highest in TYCSB agar, followed by in descending order by TYS20B, MSB, GSTB, and MSKB (p<0.01). Similar results were obtained using dental plaque samples collected from swabs of the teeth of a group of children. In the clinical samples, TYCSB again showed the highest recovery rates of S. mutans compared to the other four media. Results were reproduced at dilutions of the samples at 1:20 x 10(6) to 1:2 x 10(6), and S. mutans concentrations of 1.6 to 7.7 Log 10 CFU/mL. In addition, there were highest ratios of mutans to non-mutans bacteria in TYCSB compared to the other media, suggesting high selectivity of this media for mutans species. CONCLUSION: The results of our study suggest that TYCSB is the most sensitive and selective media for culture of S. mutans for laboratory and clinical studies.
Authors: Kyounga Cheon; Stephen A Moser; Howard W Wiener; Jennifer Whiddon; Stephanie S Momeni; John D Ruby; Gary R Cutter; Noel K Childers Journal: Eur J Oral Sci Date: 2013-04-19 Impact factor: 2.612
Authors: Noel K Childers; Robert C Osgood; Kuei-Ling Hsu; Chanika Manmontri; Stephanie S Momeni; Harry K Mahtani; Gary R Cutter; John D Ruby Journal: Eur J Oral Sci Date: 2011-12 Impact factor: 2.612
Authors: Y Zeng; M Youssef; L Wang; N Alkhars; M Thomas; R Cacciato; S Qing; O Ly-Mapes; J Xiao Journal: J Clin Pediatr Dent Date: 2020 Impact factor: 1.065
Authors: Stephanie S Momeni; Paul Patrick; Howard W Wiener; Gary R Cutter; John D Ruby; Kyounga Cheon; Jennifer Whiddon; Stephen A Moser; Noel K Childers Journal: J Microbiol Methods Date: 2014-05-28 Impact factor: 2.363
Authors: Christopher V Hughes; Mohammed Dahlan; Eleftheria Papadopolou; Cheen Y Loo; Nooruddin S Pradhan; Shulin C Lu; Jennifer M J Mathney; Alexandra Bravoco; Ralph L Kent; Anne C R Tanner Journal: Pediatr Dent Date: 2012 Mar-Apr Impact factor: 1.874