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Literature DB >> 12034814

Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G40P, interacts with forked DNA.

Silvia Ayora¹, Frank Weise, Pablo Mesa, Andrzej Stasiak, Juan C Alonso.

Abstract

SPP1-encoded replicative DNA helicase gene 40 product (G40P) is an essential product for phage replication. Hexameric G40P, in the presence of AMP-PNP, preferentially binds unstructured single-stranded (ss)DNA in a sequence-independent manner. The efficiency of ssDNA binding, nucleotide hydrolysis and the unwinding activity of G40P are affected in a different manner by different nucleotide cofactors. Nuclease protection studies suggest that G40P protects the 5' tail of a forked molecule, and the duplex region at the junction against exonuclease attack. G40P does not protect the 3' tail of a forked molecule from exonuclease attack. By using electron microscopy we confirm that the ssDNA transverses the centre of the hexameric ring. Our results show that hexameric G40P DNA helicase encircles the 5' tail, interacts with the duplex DNA at the ss-double-stranded DNA junction and excludes the 3' tail of the forked DNA.

Entities: Chemical Mutation Species

Mesh：

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Year: 2002 PMID： 12034814 PMCID： PMC117191 DOI： 10.1093/nar/30.11.2280

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

1. The 3'-tail of a forked-duplex sterically determines whether one or two DNA strands pass through the central channel of a replication-fork helicase.

Authors: D L Kaplan
Journal: J Mol Biol Date: 2000-08-11 Impact factor: 5.469

Review 2. Structure and function of hexameric helicases.

Authors: S S Patel; K M Picha
Journal: Annu Rev Biochem Date: 2000 Impact factor: 23.643

3. The Bacillus subtilis bacteriophage SPP1 G39P delivers and activates the G40P DNA helicase upon interacting with the G38P-bound replication origin.

Authors: S Ayora; A Stasiak; J C Alonso
Journal: J Mol Biol Date: 1999-04-23 Impact factor: 5.469

Review 4. Unwinding the 'Gordian knot' of helicase action.

Authors: P Soultanas; D B Wigley
Journal: Trends Biochem Sci Date: 2001-01 Impact factor: 13.807

5. Characterization of the DNA-dependent GTPase activity of T4 gene 41 protein, an essential component of the T4 bacteriophage DNA replication apparatus.

Authors: C C Liu; B M Alberts
Journal: J Biol Chem Date: 1981-03-25 Impact factor: 5.157

6. Mechanism of dnaB protein action. III. Allosteric role of ATP in the alteration of DNA structure by dnaB protein in priming replication.

Authors: K Arai; A Kornberg
Journal: J Biol Chem Date: 1981-05-25 Impact factor: 5.157

7. Bacteriophage T4 gene 41 protein, required for the synthesis of RNA primers, is also a DNA helicase.

Authors: M Venkatesan; L L Silver; N G Nossal
Journal: J Biol Chem Date: 1982-10-25 Impact factor: 5.157

8. DNA-dependent nucleoside 5'-triphosphatase activity of the gene 4 protein of bacteriophage T7.

Authors: S W Matson; C C Richardson
Journal: J Biol Chem Date: 1983-11-25 Impact factor: 5.157

9. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.

Authors: J F Scott; S Eisenberg; L L Bertsch; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

10. Mapping protein-protein interactions within a stable complex of DNA primase and DnaB helicase from Bacillus stearothermophilus.

Authors: L E Bird; H Pan; P Soultanas; D B Wigley
Journal: Biochemistry Date: 2000-01-11 Impact factor: 3.162

17 in total

Bacillus subtilis bacteriophage SPP1 hexameric DNA helicase, G40P, interacts with forked DNA.

1. The 3'-tail of a forked-duplex sterically determines whether one or two DNA strands pass through the central channel of a replication-fork helicase.

Review 2. Structure and function of hexameric helicases.

3. The Bacillus subtilis bacteriophage SPP1 G39P delivers and activates the G40P DNA helicase upon interacting with the G38P-bound replication origin.

Review 4. Unwinding the 'Gordian knot' of helicase action.

5. Characterization of the DNA-dependent GTPase activity of T4 gene 41 protein, an essential component of the T4 bacteriophage DNA replication apparatus.

6. Mechanism of dnaB protein action. III. Allosteric role of ATP in the alteration of DNA structure by dnaB protein in priming replication.

7. Bacteriophage T4 gene 41 protein, required for the synthesis of RNA primers, is also a DNA helicase.

8. DNA-dependent nucleoside 5'-triphosphatase activity of the gene 4 protein of bacteriophage T7.

9. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.

10. Mapping protein-protein interactions within a stable complex of DNA primase and DnaB helicase from Bacillus stearothermophilus.

1. Bacillus subtilis tau subunit of DNA polymerase III interacts with bacteriophage SPP1 replicative DNA helicase G40P.

Review 2. Understanding helicases as a means of virus control.

3. Bacillus subtilis RecO nucleates RecA onto SsbA-coated single-stranded DNA.

4. Molecular basis for recognition of nucleoside triphosphate by gene 4 helicase of bacteriophage T7.

5. The hexameric helicase DnaB adopts a nonplanar conformation during translocation.

6. A novel role for RecA under non-stress: promotion of swarming motility in Escherichia coli K-12.

7. Role of ATP hydrolysis in the DNA translocase activity of the bovine papillomavirus (BPV-1) E1 helicase.

8. Bacteriophage SPP1 DNA replication strategies promote viral and disable host replication in vitro.

9. Characterization of the Holliday junction resolving enzyme encoded by the Bacillus subtilis bacteriophage SPP1.

10. The RecU Holliday junction resolvase acts at early stages of homologous recombination.