Influence of calcium on the early steps of rotavirus infection.

The structure of rotaviruses and many steps of their replication cycle depend on the concentration of calcium in the microenvironment. In this work, to learn about the role of calcium during the early steps of the infection, we characterized the effect of increasing the calcium concentration in the medium on the infectivity of rotaviruses. We found that a fivefold increase in the calcium concentration of the cell culture medium results in an increased viral titer in all rotavirus strains tested. The effect of this divalent ion seems to be mainly on the viral particle and not on the surface of the cell. Analysis of the intrinsic fluorescence spectra of purified triple-layered particles revealed that changes in the environment of tryptophan residues occurred as calcium concentration increased, suggesting that conformational changes in the viral particle might be responsible for the effect of this ion on the viral infectivity.