Literature DB >> 12032695

Retrofitting of a satellite repeat DNA-based murine artificial chromosome (ACes) to contain loxP recombination sites.

S Stewart1, N MacDonald, E Perkins, G DeJong, C Perez, M Lindenbaum.   

Abstract

A satellite DNA-based mammalian artificial chromosome (ACes) was generated and subsequently modified by targeting of a loxP-red fluorescent protein (RFP) expression cassette via homologous recombination into a ribosomal DNA (rDNA)-containing locus. Clones containing correctly targeted ACes were identified by PCR from populations of RFP-expressing cells enriched by FACS sorting and were further characterized by fluorescent in situ hybridization. The targeted ACes maintained its ability to be purified to near homogeneity. Studies are currently underway to further characterize the functionality, carrying capacity, stability and transfectability of this modified ACes.

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Year:  2002        PMID： 12032695     DOI： 10.1038/sj.gt.3301757

Source DB:  PubMed          Journal:  Gene Ther        ISSN： 0969-7128            Impact factor:   5.250


  4 in total

Review 1.  Human artificial chromosomes for gene delivery and the development of animal models.

Authors:  Yasuhiro Kazuki; Mitsuo Oshimura
Journal:  Mol Ther       Date:  2011-07-12       Impact factor: 11.454

2.  A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy.

Authors:  Michael Lindenbaum; Ed Perkins; Erika Csonka; Elena Fleming; Lisa Garcia; Amy Greene; Lindsay Gung; Gyula Hadlaczky; Edmond Lee; Josephine Leung; Neil MacDonald; Alexisann Maxwell; Kathleen Mills; Diane Monteith; Carl F Perez; Joan Shellard; Sandy Stewart; Tom Stodola; Dana Vandenborre; Sandy Vanderbyl; Harry C Ledebur
Journal:  Nucleic Acids Res       Date:  2004-12-07       Impact factor: 16.971

Review 3.  A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges.

Authors:  Mitsuo Oshimura; Narumi Uno; Yasuhiro Kazuki; Motonobu Katoh; Toshiaki Inoue
Journal:  Chromosome Res       Date:  2015-02       Impact factor: 5.239

4.  Engineering Synthetic Chromosomes by Sequential Loading of Multiple Genomic Payloads over 100 Kilobase Pairs in Size.

Authors:  Amy Greene; Kara Pascarelli; Dominique Broccoli; Edward Perkins
Journal:  Mol Ther Methods Clin Dev       Date:  2019-04-29       Impact factor: 6.698
  4 in total

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