BACKGROUND: Determining the cellular content of saliva by means of conventional microscopy chamber counting is a very time-consuming and operator-sensitive procedure. This study concentrated on the use of flow cytometry to examine the cellular content of saliva. Erythrocytes, leukocytes, epithelial cells and bacteria were quantified and the results were compared with caries experience and the presence of gingivitis. METHODS: 258 uncentrifuged vortexed paraffin-stimulated saliva samples (112 males and 146 females) were analyzed with the UF-100 flow cytometer. Salivary reference values were established for erythrocyte, leukocyte, epithelial cell and bacterial count. Caries experience (DMF) and the presence of gingivitis were recorded. RESULTS: Caries experience or caries risk could not be assessed with flow cytometry. However, salivary flow cytometry may be useful in determining an individual's risk for gingivitis: a significant increase in salivary leukocytes was observed in individuals with gingivitis. At a cut-off level of 10(3) leukocytes micro l(-1) saliva, a sensitivity of 76% and a specificity of 45% was obtained. Other analytes were not significantly different between individuals with and without gingivitis. CONCLUSION: Flow cytometry of paraffin-stimulated human saliva seems a promising diagnostic or predictive tool and further investigations of diseases of the oro-pharyngeal loge, such as tonsillitis and periodontitis, should be carried out in the future.
BACKGROUND: Determining the cellular content of saliva by means of conventional microscopy chamber counting is a very time-consuming and operator-sensitive procedure. This study concentrated on the use of flow cytometry to examine the cellular content of saliva. Erythrocytes, leukocytes, epithelial cells and bacteria were quantified and the results were compared with caries experience and the presence of gingivitis. METHODS: 258 uncentrifuged vortexed paraffin-stimulated saliva samples (112 males and 146 females) were analyzed with the UF-100 flow cytometer. Salivary reference values were established for erythrocyte, leukocyte, epithelial cell and bacterial count. Caries experience (DMF) and the presence of gingivitis were recorded. RESULTS:Caries experience or caries risk could not be assessed with flow cytometry. However, salivary flow cytometry may be useful in determining an individual's risk for gingivitis: a significant increase in salivary leukocytes was observed in individuals with gingivitis. At a cut-off level of 10(3) leukocytes micro l(-1) saliva, a sensitivity of 76% and a specificity of 45% was obtained. Other analytes were not significantly different between individuals with and without gingivitis. CONCLUSION: Flow cytometry of paraffin-stimulated human saliva seems a promising diagnostic or predictive tool and further investigations of diseases of the oro-pharyngeal loge, such as tonsillitis and periodontitis, should be carried out in the future.
Authors: Karolina Elzbieta Kaczor-Urbanowicz; Yong Kim; Feng Li; Timur Galeev; Rob R Kitchen; Mark Gerstein; Kikuye Koyano; Sung-Hee Jeong; Xiaoyan Wang; David Elashoff; So Young Kang; Su Mi Kim; Kyoung Kim; Sung Kim; David Chia; Xinshu Xiao; Joel Rozowsky; David T W Wong Journal: Bioinformatics Date: 2018-01-01 Impact factor: 6.937
Authors: Peter H Rej; Madison H Bondy; Jue Lin; Aric A Prather; Brandon A Kohrt; Carol M Worthman; Dan T A Eisenberg Journal: Am J Hum Biol Date: 2020-03-18 Impact factor: 1.937
Authors: Lauren Y M Middleton; John Dou; Jonah Fisher; Jonathan A Heiss; Vy K Nguyen; Allan C Just; Jessica Faul; Erin B Ware; Colter Mitchell; Justin A Colacino; Kelly M Bakulski Journal: Epigenetics Date: 2021-02-22 Impact factor: 4.528
Authors: Alex M Garvin; Ralf Holzinger; Florian Berner; Walter Krebs; Bernhard Hostettler; Elges Lardi; Christian Hertli; Roy Quartermaine; Christoph Stamm Journal: Biomed Res Int Date: 2013-10-28 Impact factor: 3.411