Literature DB >> 12030707

Purification and characterization of an alkaline serine endopeptidase from a feather-degrading Xanthomonas maltophilia strain.

C H De Toni1, M F Richter, J R Chagas, J A P Henriques, C Termignoni.   

Abstract

A keratinolytic Xanthomonas maltophilia strain (POA-1), cultured on feather meal broth, using keratin as its sole source of carbon and nitrogen, secretes several extracellular peptidases. The major serine peptidase was purified to homogeneity by a five-step procedure. Its purity was evaluated by capillary zone electrophoresis. This enzyme has a molecular mass of 36 kDa, an optimum pH of 9.0, and an optimum temperature of 60 degrees C. The inhibitory profile using protease inhibitors shows that this enzyme is a serine endopeptidase. Besides keratin, the enzyme is active upon the substrates azokeratin, azocasein, and the following fluorogenic peptide substrates: Abz-Leu-Gly-Met-Ile-Ser-Leu-Met-Lys-Arg-Pro-Gln-EDDnp, Abz-Lys-Leu-Cys(SBzl)-Gly-Pro-Lys-Gln-EDDnp, and Abz-Lys-Pro-Cys(SBzl)-Phe-Ser-Lys-Gln-EDDnp.

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Year:  2002        PMID： 12030707     DOI： 10.1139/w02-027

Source DB:  PubMed          Journal:  Can J Microbiol        ISSN： 0008-4166            Impact factor:   2.419


  10 in total

1.  Fuzzy logic control of bioreactor for enhanced biosynthesis of alkaline protease by an alkalophilic strain of Bacillus subtilis.

Authors:  Ikram ul-Haq; Hamid Mukhtar
Journal:  Curr Microbiol       Date:  2006-01-31       Impact factor: 2.188

2.  Purification and characterization of keratinase from a new Bacillus subtilis strain.

Authors:  Cheng-gang Cai; Ji-shuang Chen; Jiong-jiong Qi; Yun Yin; Xiao-dong Zheng
Journal:  J Zhejiang Univ Sci B       Date:  2008-09       Impact factor: 3.066

3.  Isolation and characterization of a feather-degrading enzyme from Bacillus pseudofirmus FA30-01.

Authors:  Mio Kojima; Mieko Kanai; Mari Tominaga; Shunichi Kitazume; Akira Inoue; Koki Horikoshi
Journal:  Extremophiles       Date:  2006-02-18       Impact factor: 2.395

Review 4.  Biotechnological Aspects and Perspective of Microbial Keratinase Production.

Authors:  Subash C B Gopinath; Periasamy Anbu; Thangavel Lakshmipriya; Thean-Hock Tang; Yeng Chen; Uda Hashim; A Rahim Ruslinda; M K Md Arshad
Journal:  Biomed Res Int       Date:  2015-06-09       Impact factor: 3.411

5.  Sulfitolytic and keratinolytic potential of Chryseobacterium sp. RBT revealed hydrolysis of melanin containing feathers.

Authors:  Ranjit G Gurav; Jingchun Tang; Jyoti P Jadhav
Journal:  3 Biotech       Date:  2016-06-23       Impact factor: 2.406

6.  Effective multi-functional biotechnological applications of protease/keratinase enzyme produced by new Egyptian isolate (Laceyella sacchari YNDH).

Authors:  Doaa A Goda; Ahmad R Bassiouny; Nihad M Abdel Monem; Nadia A Soliman; Yasser R Abdel Fattah
Journal:  J Genet Eng Biotechnol       Date:  2020-07-02

Review 7.  Microbial Keratinases: Enzymes with Promising Biotechnological Applications.

Authors:  Beti Vidmar; Maša Vodovnik
Journal:  Food Technol Biotechnol       Date:  2018-09       Impact factor: 3.918

8.  Community-intrinsic properties enhance keratin degradation from bacterial consortia.

Authors:  Poonam Nasipuri; Jakob Herschend; Asker D Brejnrod; Jonas S Madsen; Roall Espersen; Birte Svensson; Mette Burmølle; Samuel Jacquiod; Søren J Sørensen
Journal:  PLoS One       Date:  2020-01-31       Impact factor: 3.240

9.  Production and characterization of keratinolytic protease from new wool-degrading Bacillus species isolated from Egyptian ecosystem.

Authors:  Mohamed A Hassan; Bakry M Haroun; Amro A Amara; Ehab A Serour
Journal:  Biomed Res Int       Date:  2013-07-11       Impact factor: 3.411

10.  Comparative evaluation of agroindustrial byproducts for the production of alkaline protease by wild and mutant strains of Bacillus subtilis in submerged and solid state fermentation.

Authors:  Hamid Mukhtar; Ikramul Haq
Journal:  ScientificWorldJournal       Date:  2013-10-31
  10 in total

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