Literature DB >> 12029525

MET3 promoter: a tightly regulated promoter and its application in construction of conditional lethal strain.

Xicheng Mao1, Yafang Hu, Chao Liang, Changde Lu.   

Abstract

The beta-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant ( cln1 Delta cln2 Delta pho85 Delta) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae.

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Year:  2002        PMID： 12029525     DOI： 10.1007/s00284-001-0046-0

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  14 in total

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