H Rintala1, A Nevalainen, M Suutari. 1. National Public Health Institute (KTL), Laboratory of Environmental Microbiology, Kuopio, Finland. helena.rintala@ktl.fi
Abstract
AIMS: The diversity of streptomycetes in two different types of water-damaged building materials was investigated. METHODS AND RESULTS: Direct PCR amplification of 16S rDNA from DNA isolated from building materials, cloning of the fragments and sequence analysis were used. In the phylogenetic analysis of the variable gamma region of the PCR amplification products, the sequences affiliated with five groups. CONCLUSIONS: Several different sequences were found in both materials, suggesting the presence of several species. Also, previously unknown sequences were detected, although all the sequences clustered together with sequences of known species. SIGNIFICANCE AND IMPACT OF THE STUDY: Streptomycetes are known as indicators for moisture and mould damage in buildings and potential health risk, but their diversity in indoor environments is still unknown.
AIMS: The diversity of streptomycetes in two different types of water-damaged building materials was investigated. METHODS AND RESULTS: Direct PCR amplification of 16S rDNA from DNA isolated from building materials, cloning of the fragments and sequence analysis were used. In the phylogenetic analysis of the variable gamma region of the PCR amplification products, the sequences affiliated with five groups. CONCLUSIONS: Several different sequences were found in both materials, suggesting the presence of several species. Also, previously unknown sequences were detected, although all the sequences clustered together with sequences of known species. SIGNIFICANCE AND IMPACT OF THE STUDY: Streptomycetes are known as indicators for moisture and mould damage in buildings and potential health risk, but their diversity in indoor environments is still unknown.
Authors: Elisabet Johansson; Tiina Reponen; Jarek Meller; Stephen Vesper; Jagjit Yadav Journal: Environ Monit Assess Date: 2014-10-22 Impact factor: 2.513
Authors: Balamuralikrishna Jayaprakash; Rachel I Adams; Pirkka Kirjavainen; Anne Karvonen; Asko Vepsäläinen; Maria Valkonen; Kati Järvi; Michael Sulyok; Juha Pekkanen; Anne Hyvärinen; Martin Täubel Journal: Microbiome Date: 2017-10-13 Impact factor: 14.650