| Literature DB >> 12023804 |
Alexei Kurakin1, Dale Bredesen.
Abstract
A new in vitro screening method has been developed and applied to a commercial phage-displayed cDNA library to search for novel protein-protein interactions. PDZ, WW and SH3 domains from PSD95, Nedd4, Src, Abl and Crk proteins were used as targets. 12 novel putative and 2 previously reported interactions were identified in test screens. The novel screening format, dubbed TAIS (target-assisted iterative screening), is discussed as an alternative platform to existing technologies for a pair-wise characterization of protein-protein interactions.Entities:
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Year: 2002 PMID： 12023804 DOI： 10.1080/07391102.2002.10506805
Source DB: PubMed Journal: J Biomol Struct Dyn ISSN： 0739-1102