OBJECTIVE: M-mode echocardiography has been used extensively for estimating left ventricular (LV) mass in mice, but accuracy is limited by its unidimensional nature. This study tested whether two-dimensional (2-D) echocardiographic measurement of LV mass using area-length and truncated ellipsoid methods overcomes this limitation. METHODS: Twenty-five anesthetized FVB/N mice (Mus musculus) underwent high resolution (15 MHZ) two-dimensionally directed M-mode and 2-D echocardiography. End-diastolic epicardial and endocardial areas from short-axis views and the LV orthogonal long-axis views were measured, while LV mass was calculated by 2-D area-length and truncated ellipsoid methods. LV mass was calculated from M-mode using the uncorrected cube equation. Hearts were removed and weighed after completing the echocardiographic studies. The correlation and agreement between echocardiographic-determined LV mass and gravimetric LV weight were examined. RESULTS: LV mass using the area-length method correlated better with autopsy LV weight (r = 0.87, slope = 0.87, standard error of the estimate [SEE] = 12.4 mg, P < 0.001) than M-mode echocardiography (r = 0.85, slope = 0.71, SEE = 11.1 mg, P < 0.001), and the degree of agreement (bias +/- 2 SD) was better (10.2 +/- 25.1 mg vs 13.1 +/- 25.8 mg, P = NS). Although 2-D truncated ellipsoid LV mass showed a good correlation with necropsy LV weight (r = 0.87, slope = 0.78, SEE = 11.0 mg, P < 0.001), the agreement between the truncated ellipsoid estimates and LV weight was less than that between M-mode and gravimetric LV mass. CONCLUSIONS: The 2-D area-length method is an accurate method of LV mass determination in symmetrical ventricles and can be used as an alternative for in vivo measurement of LV mass in mice.
OBJECTIVE: M-mode echocardiography has been used extensively for estimating left ventricular (LV) mass in mice, but accuracy is limited by its unidimensional nature. This study tested whether two-dimensional (2-D) echocardiographic measurement of LV mass using area-length and truncated ellipsoid methods overcomes this limitation. METHODS: Twenty-five anesthetized FVB/N mice (Mus musculus) underwent high resolution (15 MHZ) two-dimensionally directed M-mode and 2-D echocardiography. End-diastolic epicardial and endocardial areas from short-axis views and the LV orthogonal long-axis views were measured, while LV mass was calculated by 2-D area-length and truncated ellipsoid methods. LV mass was calculated from M-mode using the uncorrected cube equation. Hearts were removed and weighed after completing the echocardiographic studies. The correlation and agreement between echocardiographic-determined LV mass and gravimetric LV weight were examined. RESULTS: LV mass using the area-length method correlated better with autopsy LV weight (r = 0.87, slope = 0.87, standard error of the estimate [SEE] = 12.4 mg, P < 0.001) than M-mode echocardiography (r = 0.85, slope = 0.71, SEE = 11.1 mg, P < 0.001), and the degree of agreement (bias +/- 2 SD) was better (10.2 +/- 25.1 mg vs 13.1 +/- 25.8 mg, P = NS). Although 2-D truncated ellipsoid LV mass showed a good correlation with necropsy LV weight (r = 0.87, slope = 0.78, SEE = 11.0 mg, P < 0.001), the agreement between the truncated ellipsoid estimates and LV weight was less than that between M-mode and gravimetric LV mass. CONCLUSIONS: The 2-D area-length method is an accurate method of LV mass determination in symmetrical ventricles and can be used as an alternative for in vivo measurement of LV mass in mice.
Authors: Federico Franchi; Bruce E Knudsen; Elise Oehler; Stephen C Textor; Lilach O Lerman; Joseph P Grande; Martin Rodriguez-Porcel Journal: Hypertens Res Date: 2013-05-16 Impact factor: 3.872
Authors: Jörg Stypmann; Markus A Engelen; Constanze Epping; Harold V M van Rijen; Peter Milberg; Christian Bruch; Günter Breithardt; Klaus Tiemann; Lars Eckardt Journal: Int J Cardiovasc Imaging Date: 2006-03-04 Impact factor: 2.357
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Authors: María Valero-Muñoz; Eng Leng Saw; Ryan M Hekman; Benjamin C Blum; Zaynab Hourani; Henk Granzier; Andrew Emili; Flora Sam Journal: Front Cardiovasc Med Date: 2022-08-25
Authors: Joseph D Powers; Kristina B Kooiker; Allison B Mason; Abigail E Teitgen; Galina V Flint; Jil C Tardiff; Steven D Schwartz; Andrew D McCulloch; Michael Regnier; Jennifer Davis; Farid Moussavi-Harami Journal: JCI Insight Date: 2020-10-15