| Literature DB >> 1202241 |
H A Lewis, R C Miller, J C Stone, R A Warren.
Abstract
The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose gradients. This fragmentation was not a consequence of preexisting single-strand interruptions in the DNA, since thermal denaturation of DNA yielded intact single strands. The alpha-putrescinylthymine groups in phi W-14 DNA appeared to be labile; some, or parts of some, of these groups were cleaved from the DNA in alkali.Entities:
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Year: 1975 PMID: 1202241 PMCID: PMC355745
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103