Red blood cell rheological alterations in a rat model of ischemia-reperfusion injury.

Red blood cell (RBC) deformability and aggregation characteristics were investigated in an experimental model of ischemia-reperfusion injury. Ischemia was produced in rat hind limb by occluding the femoral artery for 10 minutes, followed by reperfusion. Blood samples were obtained either following the ischemia or 15 minutes after reperfusion. RBC deformability measured by ektacytometry was found to be significantly impaired immediately after the end of ischemic period in the blood samples obtained from femoral vein of the ischemic limb, while there was no significant difference after 15 minutes of reperfusion. In contrast, RBC aggregability was found to be decreased only after the reperfusion period and this alteration was not only limited to the blood returning from the ischemic limb but was also observed in the samples obtained from non-ischemic, contralateral hind limb, indicating a systemic alteration. RBC electrophoresis studies suggested that the altered aggregability might be related to altered RBC surface properties including increased RBC surface charge density.