Literature DB >> 12014586

Detection of mitogen-induced lymphocyte proliferation by bromodeoxyuridine (BrdU) incorporation in the chicken.

Maki Motobu1, Moshira El-Abasy, Ki-Jeong Na, Yoshikazu Hirota.   

Abstract

3H-thymidine (3H-TdR) incorporation assay has been generally used to measure lymphocyte proliferation in the chicken. Disadvantages of this assay are that radioisotope is biological hazard to the person and environment and that it can not measure which subset of lymphocytes proliferates. In this study, bromodeoxyuridine (BrdU) incorporation assay by flow cytometry was compared with 3H-TdR incorporation assay. As a result, BrdU incorporation assay showed a strong correlation with 3H-TdR incorporation assay, and it could be applied simultaneously to detect BrdU incorporation and expression of cell surface marker antigens. These results suggest that the BrdU incorporation assay by flow cytometry is useful to analyze lymphocyte proliferation in detail.

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Year:  2002        PMID： 12014586     DOI： 10.1292/jvms.64.377

Source DB:  PubMed          Journal:  J Vet Med Sci        ISSN： 0916-7250            Impact factor:   1.267


  7 in total

1.  PCNA in situ hybridization: a novel and reliable tool for detection of dynamic changes in proliferative activity.

Authors:  Thomas Köhler; Felicitas Pröls; Beate Brand-Saberi
Journal:  Histochem Cell Biol       Date:  2004-12-23       Impact factor: 2.531

2.  An EdU-based flow cytometry assay to evaluate chicken T lymphocyte proliferation.

Authors:  Karla Lucía F Alvarez; Astrid Poma-Acevedo; Manolo Fernández-Sánchez; Manolo Fernández-Díaz
Journal:  BMC Vet Res       Date:  2020-07-06       Impact factor: 2.741

3.  In vitro assessment of the feline cell-mediated immune response against feline panleukopeniavirus, calicivirus and felid herpesvirus 1 using 5-bromo-2'-deoxyuridine labeling.

Authors:  Ben L Vermeulen; Sabine E Gleich; Annelike Dedeurwaerder; Dominique A Olyslaegers; Lowiese M Desmarets; Hannah L Dewerchin; Hans J Nauwynck
Journal:  Vet Immunol Immunopathol       Date:  2012-03-10       Impact factor: 2.046

4.  Improved Whole Gamma Irradiated Avian Influenza Subtype H9N2 Virus Vaccine Using Trehalose and Optimization of Vaccination Regime on Broiler Chicken.

Authors:  Farahnaz Motamedi Sedeh; Iraj Khalili; Viskam Wijewardana; Hermann Unger; Parvin Shawrang; Mehdi Behgar; Sayed Morteza Moosavi; Arash Arbabi; Sayedeh Maede Hosseini
Journal:  Front Vet Sci       Date:  2022-07-12

Review 5.  Review: 2-mercaptoethanol alteration of in vitro immune functions of species other than murine.

Authors:  Robert E Click
Journal:  J Immunol Methods       Date:  2013-11-22       Impact factor: 2.303

Review 6.  The Evaluation of Cellular Immunity to Avian Viral Diseases: Methods, Applications, and Challenges.

Authors:  Xiaoli Hao; Fan Zhang; Yi Yang; Shaobin Shang
Journal:  Front Microbiol       Date:  2021-12-07       Impact factor: 5.640

7.  Carboxymethyl chitosan bounded iron oxide nanoparticles and gamma-irradiated avian influenza subtype H9N2 vaccine to development of immunity on mouse and chicken.

Authors:  Farahnaz Motamedi-Sedeh; Atefeh Saboorizadeh; Iraj Khalili; Massomeh Sharbatdaran; Viskam Wijewardana; Arash Arbabi
Journal:  Vet Med Sci       Date:  2021-12-08
  7 in total

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