Age-related disruptions in circadian timing: evidence for "split" activity rhythms in the SAMP8.

In the senescence-accelerated mouse of the P8 line (SAMP8), age-related changes in circadian timing include a decrease in amplitude of the rhythm, a slower rate of re-entrainment following a phase advance, and a longer free-running period (tau). The present study extends previous research by investigating possible split activity rhythms in the SAMP8. Running wheel activity was examined in 2-, 7-, and 12-month SAMP8. Consistent with previous research, rhythms of older SAMP8 were decreased in amplitude and showed high levels of activity during the light phase of the light-dark cycle. Contrary to previous reports, lengthening of tau in constant darkness was not observed. Additionally, activity rhythms were "split" in older SAMP8, demonstrated by 1) the appearance of a secondary peak in the periodogram at approximately half the value of tau and 2) the perceptual classification of actograms by naïve observers. This result differs from previous studies in hamsters demonstrating an age-related decrease in the incidence of rhythm splitting. Overall, the present findings provide further evidence for age-related disruption of circadian timing in SAMP8.