Literature DB >> 12008918

Modified pulsed-field gel electrophoresis method for DNA degradation-sensitive Salmonella enterica and Escherichia coli strains.

Almut Liesegang1, Helmut Tschäpe.   

Abstract

A number of S. enterica and E. coli strains appeared sensitive to a rapid DNA degradation during the course of PFGE pattern analysis. This kind of DNA degradation could not be stopped by intensive treatment with proteinase K, formalin treatment, or other modifications of the protocol for the isolation of intact chromosomal DNA. However, the application of 100 microM thiourea into the running buffer gave rise to clear-cut PFGE patterns and in turn to an overall typeability.

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Year:  2002        PMID： 12008918     DOI： 10.1078/1438-4221-00180

Source DB:  PubMed          Journal:  Int J Med Microbiol        ISSN： 1438-4221            Impact factor:   3.473


  11 in total

1.  Lineage-specific distribution of insertion sequence excision enhancer in enterotoxigenic Escherichia coli isolated from swine.

Authors:  Masahiro Kusumoto; Dai Fukamizu; Yoshitoshi Ogura; Eiji Yoshida; Fumiko Yamamoto; Taketoshi Iwata; Tadasuke Ooka; Masato Akiba; Tetsuya Hayashi
Journal:  Appl Environ Microbiol       Date:  2013-12-13       Impact factor: 4.792

2.  Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan.

Authors:  Masahiro Kusumoto; Yuna Hikoda; Yuki Fujii; Misato Murata; Hirotsugu Miyoshi; Yoshitoshi Ogura; Yasuhiro Gotoh; Taketoshi Iwata; Tetsuya Hayashi; Masato Akiba
Journal:  J Clin Microbiol       Date:  2016-02-10       Impact factor: 5.948

3.  Third-generation-cephalosporin-resistant Klebsiella pneumoniae isolates from humans and companion animals in Switzerland: spread of a DHA-producing sequence type 11 clone in a veterinary setting.

Authors:  Nadia Wohlwend; Andrea Endimiani; Thierry Francey; Vincent Perreten
Journal:  Antimicrob Agents Chemother       Date:  2015-03-02       Impact factor: 5.191

4.  Detection of a Salmonella enterica serovar California strain spreading in spanish feed mills and genetic characterization with DNA microarrays.

Authors:  Juan Alvarez; Steffen Porwollik; Idoia Laconcha; Vassilis Gisakis; Ana Belén Vivanco; Iratxe Gonzalez; Susana Echenagusia; Nieves Zabala; Felisa Blackmer; Michael McClelland; Aitor Rementeria; Javier Garaizar
Journal:  Appl Environ Microbiol       Date:  2003-12       Impact factor: 4.792

5.  A novel host-specific restriction system associated with DNA backbone S-modification in Salmonella.

Authors:  Tiegang Xu; Fen Yao; Xiufen Zhou; Zixin Deng; Delin You
Journal:  Nucleic Acids Res       Date:  2010-07-12       Impact factor: 16.971

6.  Prevalence and characterization of salmonellae in commercial ground beef in the United States.

Authors:  Joseph M Bosilevac; Michael N Guerini; Norasak Kalchayanand; Mohammad Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2009-02-06       Impact factor: 4.792

7.  Phosphorothioate DNA as an antioxidant in bacteria.

Authors:  Xinqiang Xie; Jingdan Liang; Tianning Pu; Fei Xu; Fen Yao; Yan Yang; Yi-Lei Zhao; Delin You; Xiufen Zhou; Zixin Deng; Zhijun Wang
Journal:  Nucleic Acids Res       Date:  2012-07-05       Impact factor: 16.971

8.  Porcine E. coli: virulence-associated genes, resistance genes and adhesion and probiotic activity tested by a new screening method.

Authors:  Peter Schierack; Stefan Rödiger; Christoph Kuhl; Rico Hiemann; Dirk Roggenbuck; Ganwu Li; Jörg Weinreich; Enrico Berger; Lisa K Nolan; Bryon Nicholson; Antje Römer; Ulrike Frömmel; Lothar H Wieler; Christian Schröder
Journal:  PLoS One       Date:  2013-04-26       Impact factor: 3.240

9.  DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites.

Authors:  Jingdan Liang; Zhijun Wang; Xinyi He; Jialiang Li; Xiufen Zhou; Zixin Deng
Journal:  Nucleic Acids Res       Date:  2007-04-16       Impact factor: 16.971

10.  Antimalarial therapy selection for quinolone resistance among Escherichia coli in the absence of quinolone exposure, in tropical South America.

Authors:  Ross J Davidson; Ian Davis; Barbara M Willey; Keyro Rizg; Shelly Bolotin; Vanessa Porter; Jane Polsky; Nick Daneman; Allison McGeer; Paul Yang; Dennis Scolnik; Roy Rowsell; Olga Imas; Michael S Silverman
Journal:  PLoS One       Date:  2008-07-16       Impact factor: 3.240
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