Effect of ethanol on axonal transport of cholinergic enzymes in rat sciatic nerve.

The aim of this study was to investigate whether the fast axonal transport of acetylcholinesterase (AChE) and the slow transport of choline acetyltransferase (ChAT) in the sciatic nerve of the rat are affected by chronic ethanol consumption or by associated nutritional deficiency. Adult rats drank ethanol (20% [vol./vol.]) instead of water for 20 weeks. Animals that consumed an isocaloric diet, representing a nutritional deficiency, received the same amount of food as ethanol-treated rats and water with sucrose replacing ethanol isocalorically. The control group received food and water ad libitum. Axonal transport was investigated by the stop-flow ligation technique as follows. After 20 weeks, the sciatic nerve was ligated for 24 h, and accumulation of AChE and ChAT was measured above and below the ligature. No significant differences in the accumulation of both enzymes were found above the ligature. However, the accumulation of AChE transported retrogradely below the nerve ligature was reduced by 60%, but only in ethanol-treated animals. Our study results seem to indicate that, under experimental conditions, (1) neither ethanol nor associated nutritional deficiency has any effect on anterograde axonal transport of AChE and ChAT and (2) the deficit in retrograde transport of AChE is due to the direct toxic effect of ethanol and probably precedes the axonal degeneration from the most distal portions of axons toward the cell body.