| Literature DB >> 12001047 |
Gerly A C Brito1, Gail W Sullivan, William P Ciesla, Holliday T Carper, Gerald L Mandell, Richard L Guerrant.
Abstract
The effects of purified toxin A in vitro on the shape and function of polymorphonuclear leukocytes (PMNL) were examined. Toxin A induced changes in adherent PMNL shape from a compact spherical or pyramidal shape to a thin and rope-like shape. This change in shape was accompanied by rearrangement of the F-actin cytoskeleton into aggregates. Toxin A-treated PMNL exhibited increased adherence and expressed less L-selectin and more Mac-1, compared with untreated PMNL. In contrast to these proinflammatory actions, toxin A impaired both directed and non-directed PMNL migration in response to N-formylmethionylleucylphenylalanine. In addition, toxin A decreased the oxidative activity of adherent PMNL stimulated by recombinant human tumor necrosis factor-alpha. These effects could be explained by toxin A-induced glucosylation of the signaling small-size guanine 5'-triphosphate-binding proteins of the Rho family in human PMNL.Entities:
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Year: 2002 PMID： 12001047 DOI： 10.1086/340236
Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226