Literature DB >> 12000852

Telomere measurement by quantitative PCR.

Richard M Cawthon1.   

Abstract

It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate investigations of the biology of telomeres and the roles they play in the molecular pathophysiology of diseases and aging.

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Year:  2002        PMID： 12000852      PMCID： PMC115301          DOI： 10.1093/nar/30.10.e47

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Telomere end-replication problem and cell aging.

Authors:  M Z Levy; R C Allsopp; A B Futcher; C W Greider; C B Harley
Journal:  J Mol Biol       Date:  1992-06-20       Impact factor: 5.469

2.  Heterogeneity in telomere length of human chromosomes.

Authors:  P M Lansdorp; N P Verwoerd; F M van de Rijke; V Dragowska; M T Little; R W Dirks; A K Raap; H J Tanke
Journal:  Hum Mol Genet       Date:  1996-05       Impact factor: 6.150

3.  Telomere analysis by fluorescence in situ hybridization and flow cytometry.

Authors:  M Hultdin; E Grönlund; K Norrback; E Eriksson-Lindström; T Just; G Roos
Journal:  Nucleic Acids Res       Date:  1998-08-15       Impact factor: 16.971

4.  Sensitive method for measuring telomere lengths by quantifying telomeric DNA content of whole cells.

Authors:  D Norwood; D S Dimitrov
Journal:  Biotechniques       Date:  1998-12       Impact factor: 1.993

5.  Measurement of telomeric DNA content in human tissues.

Authors:  J E Bryant; K G Hutchings; R K Moyzis; J K Griffith
Journal:  Biotechniques       Date:  1997-09       Impact factor: 1.993

6.  Construction of linkage maps with DNA markers for human chromosomes.

Authors:  R White; M Leppert; D T Bishop; D Barker; J Berkowitz; C Brown; P Callahan; T Holm; L Jerominski
Journal:  Nature       Date:  1985 Jan 10-18       Impact factor: 49.962

7.  Human telomeres contain at least three types of G-rich repeat distributed non-randomly.

Authors:  R C Allshire; M Dempster; N D Hastie
Journal:  Nucleic Acids Res       Date:  1989-06-26       Impact factor: 16.971

8.  Telomeres shorten during ageing of human fibroblasts.

Authors:  C B Harley; A B Futcher; C W Greider
Journal:  Nature       Date:  1990-05-31       Impact factor: 49.962

9.  Genetic determination of telomere size in humans: a twin study of three age groups.

Authors:  P E Slagboom; S Droog; D I Boomsma
Journal:  Am J Hum Genet       Date:  1994-11       Impact factor: 11.025

10.  Kinetic PCR analysis: real-time monitoring of DNA amplification reactions.

Authors:  R Higuchi; C Fockler; G Dollinger; R Watson
Journal:  Biotechnology (N Y)       Date:  1993-09
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  1358 in total

1.  Shortened telomeres in circulating leukocytes of patients with chronic obstructive pulmonary disease.

Authors:  Laurent Savale; Ari Chaouat; Sylvie Bastuji-Garin; Elisabeth Marcos; Laurent Boyer; Bernard Maitre; Mourad Sarni; Bruno Housset; Emmanuel Weitzenblum; Mireille Matrat; Philippe Le Corvoisier; Dominique Rideau; Jorge Boczkowski; Jean-Luc Dubois-Randé; Christos Chouaid; Serge Adnot
Journal:  Am J Respir Crit Care Med       Date:  2009-01-29       Impact factor: 21.405

2.  Telomere length in patients with systemic lupus erythematosus and its associations with carotid plaque.

Authors:  Carly Skamra; Juanita Romero-Diaz; Alexander Sandhu; QiQuan Huang; Jungwha Lee; William Pearce; David D McPherson; Kim Sutton-Tyrrell; Richard Pope; Rosalind Ramsey-Goldman
Journal:  Rheumatology (Oxford)       Date:  2013-02-04       Impact factor: 7.580

3.  Telomere length as a risk factor for hereditary prostate cancer.

Authors:  Lauren M Hurwitz; Christopher M Heaphy; Corinne E Joshu; William B Isaacs; Yuko Konishi; Angelo M De Marzo; Sally D Isaacs; Kathy E Wiley; Elizabeth A Platz; Alan K Meeker
Journal:  Prostate       Date:  2013-11-28       Impact factor: 4.104

4.  Leukocyte telomere length and ideal cardiovascular health in American Indians: the Strong Heart Family Study.

Authors:  Hao Peng; Mihriye Mete; Sameer Desale; Amanda M Fretts; Shelley A Cole; Lyle G Best; Jue Lin; Elizabeth Blackburn; Elisa T Lee; Barbara V Howard; Jinying Zhao
Journal:  Eur J Epidemiol       Date:  2016-09-22       Impact factor: 8.082

5.  PAI-1-regulated extracellular proteolysis governs senescence and survival in Klotho mice.

Authors:  Mesut Eren; Amanda E Boe; Sheila B Murphy; Aaron T Place; Varun Nagpal; Luisa Morales-Nebreda; Daniela Urich; Susan E Quaggin; G R Scott Budinger; Gökhan M Mutlu; Toshio Miyata; Douglas E Vaughan
Journal:  Proc Natl Acad Sci U S A       Date:  2014-04-28       Impact factor: 11.205

6.  Accelerated telomere shortening in response to life stress.

Authors:  Elissa S Epel; Elizabeth H Blackburn; Jue Lin; Firdaus S Dhabhar; Nancy E Adler; Jason D Morrow; Richard M Cawthon
Journal:  Proc Natl Acad Sci U S A       Date:  2004-12-01       Impact factor: 11.205

7.  Elevated plasma magnesium and calcium may be associated with shorter telomeres in older South Australian women.

Authors:  N J O'Callaghan; C Bull; M Fenech
Journal:  J Nutr Health Aging       Date:  2014       Impact factor: 4.075

8.  Telomere length and pancreatic cancer: a case-control study.

Authors:  Halcyon G Skinner; Ronald E Gangnon; Kristin Litzelman; Ruth A Johnson; Suresh T Chari; Gloria M Petersen; Lisa A Boardman
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  2012-10-23       Impact factor: 4.254

9.  Aberrant Telomere Length in Circulating Cell-Free DNA as Possible Blood Biomarker with High Diagnostic Performance in Endometrial Cancer.

Authors:  Marco Benati; Martina Montagnana; Elisa Danese; Martina Mazzon; Elisa Paviati; Simone Garzon; Antonio Simone Laganà; Jvan Casarin; Silvia Giudici; Ricciarda Raffaelli; Fabio Ghezzi; Massimo Franchi; Giuseppe Lippi
Journal:  Pathol Oncol Res       Date:  2020-05-27       Impact factor: 3.201

10.  Major depressive disorder and accelerated cellular aging: results from a large psychiatric cohort study.

Authors:  J E Verhoeven; D Révész; E S Epel; J Lin; O M Wolkowitz; B W J H Penninx
Journal:  Mol Psychiatry       Date:  2013-11-12       Impact factor: 15.992
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