| Literature DB >> 11992820 |
Mansour Karimi1, Dirk Inzé, Ann Depicker.
Abstract
Agrobacterium tumefaciens is the preferred method for transformation of a wide range of plant species. Commonly, the genes to be transferred are cloned between the left and right T-DNA borders of so-called binary T-DNA vectors that can replicate both in E. coli and Agrobacterium. Because these vectors are generally large, cloning can be time-consuming and laborious. Recently, the GATEWAY conversion technology has provided a fast and reliable alternative to the cloning of sequences into large acceptor plasmids.Entities:
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Year: 2002 PMID： 11992820 DOI： 10.1016/s1360-1385(02)02251-3
Source DB: PubMed Journal: Trends Plant Sci ISSN： 1360-1385 Impact factor: 18.313