OBJECTIVE: To study the genotype of HCV epidemic strains in Shandong Province. METHODS: HCV fragments of C(432 bp) and NS5(319 bp) regions were amplified by RT nested PCR. The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction. RESULTS: Three of all 4 strains in C region were 1b genotype, and 1 was 2a. All of 10 strains of NS5 region were 1b genotype. No other genotype was found in this region. The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in Gen Bank. CONCLUSIONS: The major genotype of HCV epidemic strains in Shandong Province is 1b. The 2a genotype is also found in this area.
OBJECTIVE: To study the genotype of HCV epidemic strains in Shandong Province. METHODS: HCV fragments of C(432 bp) and NS5(319 bp) regions were amplified by RT nested PCR. The fragments were cloned to T vector and sequenced by dideoxynucleotide chain termination reaction. RESULTS: Three of all 4 strains in C region were 1b genotype, and 1 was 2a. All of 10 strains of NS5 region were 1b genotype. No other genotype was found in this region. The homology of nucleotide sequence was over 90% compared with that of more than 50 strains in Gen Bank. CONCLUSIONS: The major genotype of HCV epidemic strains in Shandong Province is 1b. The 2a genotype is also found in this area.