Literature DB >> 11986263

Optimization of a human papillomavirus-specific enzyme-linked immunosorbent assay.

Kevin L Karem1, Alysia C Poon, Cynthia Bierl, Rosane Nisenbaum, Elizabeth Unger.   

Abstract

A strategy was developed for the control, standardization, and critical evaluation of an enzyme-linked immunosorbent assay (ELISA) for the detection of human papillomavirus-specific immunoglobulin G in human sera. Control human sera, polyclonal animal sera, and monoclonal antibodies were used to establish optimal assay parameters, including antigen coating, serum dilutions, and criteria for daily reproducibility, monitoring, and rejection of assays. Three evaluation techniques were used in parallel to define an optimal cutoff absorbance value that yields greater than 93% sensitivity and 98.5% specificity in the assay's ability to discriminate positive and negative control sera. This strategy provides an optimal method by which to determine cutoff absorbance values for ELISA.

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Year:  2002        PMID： 11986263      PMCID： PMC120007          DOI： 10.1128/cdli.9.3.577-582.2002

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


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