Literature DB >> 11983939

Harvesting cells under anchorage-independent cell transformation conditions for biochemical analyses.

Zigang Dong1, Joan L Cmarik.   

Abstract

Most molecular biology and biochemical analyses use cultured cells grown in anchorage-dependent monolayer conditions. The standard oncogenic transformation assay for cell lines is usually performed in soft agar rather than in monolayers because of the higher transformation efficiency of cells in soft agar. However, cells suspended in soft agar cannot be readily recovered for studying inducible biochemical and molecular events. We developed an over-agar assay that enables us to study tumor promoter-induced cell transformation and the associated biochemical or molecular events under anchorage-independent conditions.

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Year:  2002        PMID： 11983939     DOI： 10.1126/stke.2002.130.pl7

Source DB:  PubMed          Journal:  Sci STKE        ISSN： 1525-8882


  18 in total

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Journal:  Pharm Res       Date:  2006-12-05       Impact factor: 4.200

Review 2.  Molecular and cellular targets.

Authors:  Ann M Bode; Zigang Dong
Journal:  Mol Carcinog       Date:  2006-06       Impact factor: 4.784

3.  Phosphorylation of histone H3 at serine 10 is indispensable for neoplastic cell transformation.

Authors:  Hong Seok Choi; Bu Young Choi; Yong-Yeon Cho; Hideya Mizuno; Bong Seok Kang; Ann M Bode; Zigang Dong
Journal:  Cancer Res       Date:  2005-07-01       Impact factor: 12.701

4.  Migfilin interacts with Src and contributes to cell-matrix adhesion-mediated survival signaling.

Authors:  Jianping Zhao; Yongjun Zhang; Sujay Subbayya Ithychanda; Yizeng Tu; Ka Chen; Jun Qin; Chuanyue Wu
Journal:  J Biol Chem       Date:  2009-10-15       Impact factor: 5.157

5.  The anticancer effects of actinoporin RTX-A from the sea anemone Heteractis crispa (=Radianthus macrodactylus).

Authors:  Sergey Fedorov; Sergey Dyshlovoy; Margarita Monastyrnaya; Larisa Shubina; Elena Leychenko; Emma Kozlovskaya; Jun-O Jin; Jong-Young Kwak; Ann M Bode; Zigang Dong; Valentin Stonik
Journal:  Toxicon       Date:  2009-11-26       Impact factor: 3.033

6.  The resveratrol analogue 3,5,3',4',5'-pentahydroxy-trans-stilbene inhibits cell transformation via MEK.

Authors:  Ki Won Lee; Nam Joo Kang; Evgeny A Rogozin; Sang-Muk Oh; Yong Seok Heo; Angelo Pugliese; Ann M Bode; Hyong Joo Lee; Zigang Dong
Journal:  Int J Cancer       Date:  2008-12-01       Impact factor: 7.396

7.  Marine two-headed sphingolipid-like compound rhizochalin inhibits EGF-induced transformation of JB6 P+ Cl41 cells.

Authors:  Sergey N Fedorov; Tatyana N Makarieva; Alla G Guzii; Larisa K Shubina; Jong Y Kwak; Valentin A Stonik
Journal:  Lipids       Date:  2009-07-05       Impact factor: 1.880

8.  The P110 subunit of PI3-K is a therapeutic target of acacetin in skin cancer.

Authors:  Sung Keun Jung; Jong Eun Kim; Sung-Young Lee; Mee Hyun Lee; Sanguine Byun; Young A Kim; Tae Gyu Lim; Kanamata Reddy; Zunnan Huang; Ann M Bode; Hyong Joo Lee; Ki Won Lee; Zigang Dong
Journal:  Carcinogenesis       Date:  2013-08-02       Impact factor: 4.944

9.  Cocoa procyanidins suppress transformation by inhibiting mitogen-activated protein kinase kinase.

Authors:  Nam Joo Kang; Ki Won Lee; Dong Eun Lee; Evgeny A Rogozin; Ann M Bode; Hyong Joo Lee; Zigang Dong
Journal:  J Biol Chem       Date:  2008-06-02       Impact factor: 5.157

10.  Inhibitory effects of caffeine analogues on neoplastic transformation: structure-activity relationship.

Authors:  Evgeny A Rogozin; Ki Won Lee; Nam Joo Kang; Haoyu Yu; Masaaki Nomura; Ken-Ichi Miyamoto; Allan H Conney; Ann M Bode; Zigang Dong
Journal:  Carcinogenesis       Date:  2008-01-14       Impact factor: 4.944
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