| Literature DB >> 1197784 |
Abstract
A method is described which measures the four main prostaglandins of human semen (PGE1, E2, 19-hydroxy PGE1, and 19-hydroxy PGE2). For routine measurements E1 and E2 are measured together as are 19-OH E1 and 19-OH E2. These are measured by forming oximes in aqueous solution extraction, methylation and trimethyl silylation followed by gas chromatography. The method has sufficient sensitivity to measure the levels found in the majority of semen samples. The normal range in men with proven fertility was 90 to 260 mug/ml of 19-hydroxy Es and 30-200 mug/ml of Es.Entities:
Keywords: Biology; Clinical Research; Endocrine System; Examinations And Diagnoses; Genitalia; Genitalia, Male; Laboratory Examinations And Diagnoses; Laboratory Procedures; Physiology; Prostaglandins--analysis; Research Methodology; Semen; Seminal Vesicles; Urogenital System
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Year: 1975 PMID： 1197784 DOI： 10.1016/0090-6980(75)90131-8
Source DB: PubMed Journal: Prostaglandins ISSN： 0090-6980