Literature DB >> 11968055

Evaluation of a single-step multiplex RT-PCR for influenza virus type and subtype detection in respiratory samples.

Saibal K Poddar1, Rowena Espina, David P Schnurr.   

Abstract

A single-step multiplex reverse transcription-polymerase chain reaction (RT-PCR) for the simultaneous detection of influenza virus type and subtypes was evaluated for its use in clinical specimens. One part of each specimen was tested using an established standard culture and/or monoclonal antibody-based immunofluorescence assay method, and the other part was tested by the multiplex RT-PCR method for the presence or absence of the influenza virus, and its type and subtype. Sixty-seven specimens were examined. The results revealed a strong agreement between the data obtained by the established method and those obtained by the multiplex RT-PCR. Copyright 2002 Wiley-Liss, Inc.

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Year:  2002        PMID： 11968055      PMCID： PMC6807991          DOI： 10.1002/jcla.10036

Source DB:  PubMed          Journal:  J Clin Lab Anal        ISSN： 0887-8013            Impact factor:   2.352


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