Activation of spinal extracellular signaling-regulated kinase-1 and -2 by intraplantar carrageenan in rodents.

We have examined the activation of spinal extracellular signaling-regulated kinase (ERK) in juvenile rats and adult mice after intraplantar carrageenan or saline and its relationship to pain behavior. In rats, intraplantar carrageenan evoked a peak five-fold activation of spinal ERK at 30 min measured by immunoblot. Saline injection resulted in a two-fold activation. This differential ERK activation correlated with a 2.5-fold greater pain response and the development of secondary hyperalgesia in carrageenan-injected rats, whereas both saline and carrageenan produced similar primary hyperalgesia. In mice, carrageenan injection produced a peak 3.5-fold activation of ERK, but saline was ineffective. We conclude that ERK activation may underlie spinal nociceptive processing and secondary hyperalgesia after carrageenan inflammation.