OBJECTIVE: To investigate the expression of RhoA mRNA in colorectal neoplasms. METHODS: Total RNA was extracted from 42 surgically resected specimens of colorectal caricinoma and normal mucosa close to the lesions. Afier the optimal reverse transcription polymerase chain reaction (RT-PCR) condition was found, the mRNA expression levels of the RhoA gene in the lesions and the normal mucosa were examined semi-quantitatively and the relation between these expression levels and clinical pathological markers was compared. RESULTS: The expression of RhoA gene mRNA in lesion of colorectal carcinoma was significantly higher than that in contiguous normal mucosa (1.8 +/- 0.6 vs 0.9 +/- 0.4, P < 0.01). The ratio of RhoA expression in neoplasm to that in contiguous mucosa was 2.02 +/- 0.82. Based on this ratio the 42 patients were divided into two groups: overexpression group (19 cases) and high-expression group (23 cases). In the overexpression group the lesions of 14 cases were in Dukes C and Dukes D stages, and lymph node metastasis was found in 13 cases. In the high-expression group, only 9 out of the 23 cases were in advanced stage and 8 cases showed lymph-node metastasis. Chi-squared test analysis demonstrated a significant difference in both aspects between these two groups (P < 0.05). The difference in number of metastatic lymph nodes between the two groups was statistically significant (P < 0.01). Mutation was not found in the RhoA gene sequence examined. CONCLUSION: RhoA gene may play an important role in colorectal carcinogenesis and correlate with invasion and metastasis of colorectal carcinoma.
OBJECTIVE: To investigate the expression of RhoA mRNA in colorectal neoplasms. METHODS: Total RNA was extracted from 42 surgically resected specimens of colorectal caricinoma and normal mucosa close to the lesions. Afier the optimal reverse transcription polymerase chain reaction (RT-PCR) condition was found, the mRNA expression levels of the RhoA gene in the lesions and the normal mucosa were examined semi-quantitatively and the relation between these expression levels and clinical pathological markers was compared. RESULTS: The expression of RhoA gene mRNA in lesion of colorectal carcinoma was significantly higher than that in contiguous normal mucosa (1.8 +/- 0.6 vs 0.9 +/- 0.4, P < 0.01). The ratio of RhoA expression in neoplasm to that in contiguous mucosa was 2.02 +/- 0.82. Based on this ratio the 42 patients were divided into two groups: overexpression group (19 cases) and high-expression group (23 cases). In the overexpression group the lesions of 14 cases were in Dukes C and Dukes D stages, and lymph node metastasis was found in 13 cases. In the high-expression group, only 9 out of the 23 cases were in advanced stage and 8 cases showed lymph-node metastasis. Chi-squared test analysis demonstrated a significant difference in both aspects between these two groups (P < 0.05). The difference in number of metastatic lymph nodes between the two groups was statistically significant (P < 0.01). Mutation was not found in the RhoA gene sequence examined. CONCLUSION:RhoA gene may play an important role in colorectal carcinogenesis and correlate with invasion and metastasis of colorectal carcinoma.
Authors: Haibo Wang; Gang Zhao; Xiangping Liu; Aihua Sui; Kun Yang; Ruyong Yao; Zongbao Wang; Qiang Shi Journal: J Exp Clin Cancer Res Date: 2010-09-09