| Literature DB >> 11948856 |
I A Kozlov1, S Mao, Y Xu, X Huang, L Lee, P S Sears, C Gao, A R Coyle, K D Janda, C H Wong.
Abstract
We introduced a novel method, through mirror-image phage display, for the identification of high-affinity D-peptides to target specific cell-surface carbohydrates. Both 3-deoxy-alpha-L-manno-2-octulosonic acid (L-KDO) and L-sialic acid and an L-sialo-disaccharide have been synthesized and attached to a solid support for selection of high-affinity peptide binders displayed on phages. Our initial studies in this effort produce single-chain Fab sequences and dodecapeptides that bind to sialic acid and KDO with nanomolar and high micromolar affinity.Entities:
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Year: 2001 PMID： 11948856 DOI： 10.1002/1439-7633(20011001)2:10<741::AID-CBIC741>3.0.CO;2-B
Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164