| Literature DB >> 11944920 |
Heinz Troxler1, Frank Neuheiser, Peter Kleinert, Thomas Kuster, Claus W Heizmann, Ragna Sack, Peter Hunziker, Thomas J Neuhaus, Marlis Schmid, Hannes Frischknecht.
Abstract
A novel hemoglobin variant was detected by electrospray ionization mass spectrometry. Hb Zurich-Hottingen is characterized by an Asn --> Ser replacement in the alpha-chain at position 9 as confirmed by DNA analysis. This hemoglobin variant is silent in isoelectric focusing, reversed-phase chromatography, and cation-exchange chromatography. The mutant alpha-chain was detectable only with electrospray mass spectrometry by its mass shift of -27 Da. The carrier was found to be heterozygous for the new hemoglobin variant. These results illustrate the power of ESI mass spectrometry for hemoglobin analysis. (c)2002 Elsevier Science (USA).Entities:
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Year: 2002 PMID： 11944920 DOI： 10.1006/bbrc.2002.6762
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575