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Literature DB >> 1194239

Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.

Abstract

The quantity of penicillinase plasmid deoxyribonucleic acid (DNA) in various strains of Staphylococcus aureus has been determined by DNA-DNA reassociation kinetics. Specifically, 32P- or 125I-labeled denatured probes of purified plasmid DNA were reassociated in the presence of denatured DNAs isolated from the bacterial strains in question. The number of plasmid copies per cell was calculated from the effect of the latter nucleic acid samples on the reassociation rate of the radiolabeled probe. Among the S. aureus strains examined were monoplasmid, diplasmid and replication-defective representatives, and the effect of temperature on wild-type plasmid content was also investigated.

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Year: 1975 PMID： 1194239 PMCID： PMC236055 DOI： 10.1128/jb.124.3.1417-1423.1975

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

21 in total

1. Replication of R-factor R1 in Scherichia coli K-12 at different growth rates.

Authors: B Engberg; K Nordström
Journal: J Bacteriol Date: 1975-07 Impact factor: 3.490

Review 2. Molecular structure of bacterial plasmids.

Authors: R C Clowes
Journal: Bacteriol Rev Date: 1972-09

3. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.

Authors: M Nonoyama; J S Pagano
Journal: Nature Date: 1973-03-02 Impact factor: 49.962

4. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes.

Authors: L D Gelb; D E Kohne; M A Martin
Journal: J Mol Biol Date: 1971-04-14 Impact factor: 5.469

5. Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature.

Authors: E H Asheshov
Journal: J Gen Microbiol Date: 1966-03

6. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.

Authors: R Novick
Journal: Virology Date: 1967-09 Impact factor: 3.616

7. Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.

Authors: R P Novick
Journal: Mol Gen Genet Date: 1974

8. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.

Authors: R P Novick; R Brodsky
Journal: J Mol Biol Date: 1972-07-21 Impact factor: 5.469

9. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.

Authors: P A Sharp; U Pettersson; J Sambrook
Journal: J Mol Biol Date: 1974-07-15 Impact factor: 5.469

Detection and quantitation of Staphylococcus aureus penicillinase plasmid deoxyribonucleic acid by reassociation kinetics.

1. Replication of R-factor R1 in Scherichia coli K-12 at different growth rates.

Review 2. Molecular structure of bacterial plasmids.

3. Homology between Epstein-Barr virus DNA and viral DNA from Burkitt's lymphoma and nasopharyngeal carcinoma determined by DNA-DNA reassociation kinetics.

4. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes.

5. Loss of antibiotic resistance in Staphylococcus aureus resulting from growth at high temperature.

6. Properties of a cryptic high-frequency transducing phage in Staphylococcus aureus.

7. Studies on plasmid replication. III. Isolation and characterization of replication-defective mutants.

8. Studies on plasmid replication. I. Plasmid incompatibility and establishment in Staphylococcus aureus.

9. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome.

10. Mutations in R factors of Escherichia coli causing an increased number of R-factor copies per chromosome.

1. Accessory gene regulator control of staphyloccoccal enterotoxin d gene expression.

2. Nucleic acid hybridization analysis of an integrated plasmid in Staphylococcus aureus.

3. Control of plasmid R6K copy numbers in isogenic rep+ and rep Escherichia coli strains.

4. A simple and generally applicable procedure for releasing DNA from bacterial cells.

5. Coding sequence for the pT181 repC product: a plasmid-coded protein uniquely required for replication.

6. Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus.