OBJECTIVE: To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age. METHODS: Enucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry. RESULTS: bFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P < 0.05). CONCLUSION: bFGF might play an important role in the proliferation of residual human cataract LECs after cataract surgery.
OBJECTIVE: To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of humancataract lens epithelial cells (LECs) and its correlation with age. METHODS: Enucleated eyes were subjected to immunostaining for bFGF protein. Humancataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry. RESULTS:bFGF protein was found in various human ocular tissues. bFGF stimulated humancataractLEC proliferation, and there was an age-related decrease in responsiveness of humancataract LECs to bFGF (P < 0.05). CONCLUSION:bFGF might play an important role in the proliferation of residual humancataract LECs after cataract surgery.
Authors: Bei Liu; Jing Gao; Bo-Chang Lyu; Shan-Shuang Du; Cheng Pei; Zhong-Qiao Zhu; Bo Ma Journal: Int J Ophthalmol Date: 2017-07-18 Impact factor: 1.779
Authors: Umesh C S Yadav; Farshid Ighani-Hosseinabad; Frederik J G M van Kuijk; Satish K Srivastava; Kota V Ramana Journal: Invest Ophthalmol Vis Sci Date: 2008-11-14 Impact factor: 4.799