B G H Schoser1, D Blottner, H-J Stuerenburg. 1. Friedrich-Baur-Institute, Department of Neurology, Ludwig-Maximillians University Munich, Munich, Germany. benedikt.schoser@fbs.med.uni-muenchen.de
Abstract
OBJECTIVES: To further examine the role of proteolytic enzyme expression of matrix metalloproteinases (MMP) and T-cell markers in inflammatory myopathies and controls. MATERIAL AND METHODS: We studied the expression of MMP-2, MMP-7, and MMP-9 in 19 cases of inflammatory myopathies and controls using immunocytochemistry. RESULTS: Inflammatory myopathies showed distinct patterns of up-regulation of MMP. MMP-9 was strongly expressed in atrophic myofibers in all inflammatory myopathies. MMP-2 immunoreactivity was similar in its distribution, however, to a weaker intensity. In dermatomyositis the perifascicular atrophy showed pronounced MMP-9 immunoreactivity, probably reflecting denervated patterns of myofibers. Moreover, MMP-7 strongly immunolabeled invaded myofibers in polymyositis cases only. CONCLUSION: These patterns confirm, that MMP-7 up-regulation is prominent in PM, while MMP-2 immunoreactivity is only slightly elevated in inflamed muscle. In general, MMP-9 up-regulation appears to be an important additional molecular event in the multistep process of all inflammatory myopathies.
OBJECTIVES: To further examine the role of proteolytic enzyme expression of matrix metalloproteinases (MMP) and T-cell markers in inflammatory myopathies and controls. MATERIAL AND METHODS: We studied the expression of MMP-2, MMP-7, and MMP-9 in 19 cases of inflammatory myopathies and controls using immunocytochemistry. RESULTS:Inflammatory myopathies showed distinct patterns of up-regulation of MMP. MMP-9 was strongly expressed in atrophic myofibers in all inflammatory myopathies. MMP-2 immunoreactivity was similar in its distribution, however, to a weaker intensity. In dermatomyositis the perifascicular atrophy showed pronounced MMP-9 immunoreactivity, probably reflecting denervated patterns of myofibers. Moreover, MMP-7 strongly immunolabeled invaded myofibers in polymyositis cases only. CONCLUSION: These patterns confirm, that MMP-7 up-regulation is prominent in PM, while MMP-2 immunoreactivity is only slightly elevated in inflamed muscle. In general, MMP-9 up-regulation appears to be an important additional molecular event in the multistep process of all inflammatory myopathies.
Authors: Malgorzata Zimowska; Krzysztof H Olszynski; Marta Swierczynska; Wladyslawa Streminska; Maria A Ciemerych Journal: Tissue Eng Part A Date: 2012-04-20 Impact factor: 3.845
Authors: Alexandra C Braun; Marcus Gutmann; Regina Ebert; Franz Jakob; Henning Gieseler; Tessa Lühmann; Lorenz Meinel Journal: Pharm Res Date: 2016-09-14 Impact factor: 4.200
Authors: Molly C Madden; William C Byrnes; Jacob A Lebin; Matthew E Batliner; David L Allen Journal: Eur J Appl Physiol Date: 2011-01-11 Impact factor: 3.078
Authors: Apurva K Srivastava; Xuezhong Qin; Nia Wedhas; Marc Arnush; Thomas A Linkhart; Robert B Chadwick; Ashok Kumar Journal: J Biol Chem Date: 2007-09-26 Impact factor: 5.157