Literature DB >> 11937680

Genes encoding chimeras of Neurospora crassa erg-3 and human TM7SF2 proteins fail to complement Neurospora and yeast sterol C-14 reductase mutants.

A Prakash1, Durgadas P Kasbekar.   

Abstract

The human gene TM7SF2 encodes a polypeptide (SR-1) with high sequence similarity to sterol C-14 reductase, a key sterol biosynthetic enzyme in fungi, plants and mammals. In Neurospora and yeast this enzyme is encoded by the erg-3 and erg24 genes respectively. In an effort to demonstrate sterol C-14 reductase activity for SR-1 we constructed six recombinant genes coding for chimeras of the Neurospora erg-3 and SR-1 protein sequences and tested them for complementation of the Neurospora erg-3 mutant. To our surprise, all the chimeras failed to complement erg-3. A few of the chimeric proteins were also tested against the yeast erg24 mutant, but again there was no complementation. We discuss some reasons that might account for these unexpected findings.

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Year:  2002        PMID： 11937680     DOI： 10.1007/BF02703766

Source DB:  PubMed          Journal:  J Biosci        ISSN： 0250-5991            Impact factor:   1.826


  16 in total

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Journal:  Nucleic Acids Res       Date:  1992-03-25       Impact factor: 16.971

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Authors:  D D Perkins; A Radford; D Newmeyer; M Björkman
Journal:  Microbiol Rev       Date:  1982-12

5.  The sterol C-14 reductase encoded by the Neurospora crassa erg-3 gene: essential charged and polar residues identified by site-specific mutagenesis.

Authors:  A Prakash; D P Kasbekar
Journal:  Mol Genet Genomics       Date:  2001-10-16       Impact factor: 3.291

6.  Increasing expression of P450 and P450-reductase proteins from monocots in heterologous systems.

Authors:  Y Batard; A Hehn; S Nedelkina; M Schalk; K Pallett; H Schaller; D Werck-Reichhart
Journal:  Arch Biochem Biophys       Date:  2000-07-01       Impact factor: 4.013

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Authors:  N N Pandit; V E Russo
Journal:  Mol Gen Genet       Date:  1992-09

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Authors:  Q Ye; H J Worman
Journal:  J Biol Chem       Date:  1994-04-15       Impact factor: 5.157

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Authors:  D Mumberg; R Müller; M Funk
Journal:  Gene       Date:  1995-04-14       Impact factor: 3.688

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Authors:  J H Crowley; S J Smith; F W Leak; L W Parks
Journal:  J Bacteriol       Date:  1996-05       Impact factor: 3.490
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  4 in total

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Authors:  Ashwin Bhat; Ranjan Tamuli; Durgadas P Kasbekar
Journal:  Genetics       Date:  2004-07       Impact factor: 4.562

2.  Benign anomaly to malign dysplasia: variable expression of lamin B receptor mutations in humans.

Authors:  Durgadas P Kasbekar
Journal:  J Biosci       Date:  2004-12       Impact factor: 1.826

3.  Twin enzymes, divergent control: The cholesterogenic enzymes DHCR14 and LBR are differentially regulated transcriptionally and post-translationally.

Authors:  Isabelle M Capell-Hattam; Laura J Sharpe; Lydia Qian; Gene Hart-Smith; Anika V Prabhu; Andrew J Brown
Journal:  J Biol Chem       Date:  2020-01-07       Impact factor: 5.157

4.  Lymphohematopoietic licence: sterol C-14 reductase activity of lamin B receptor (Lbr) is essential for neutrophil differentiation.

Authors:  Durgadas P Kasbekar
Journal:  J Biosci       Date:  2012-06       Impact factor: 1.826
  4 in total

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