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Literature DB >> 11936253

Magnetite-alginate beads for purification of some starch degrading enzymes.

Abstract

Starch degrading enzymes, viz., beta-amylase, glucoamylase, and pullulanase, were purified using magnetite-alginate beads. In each case, the enzyme activity was eluted by using 1.0 M maltose. beta-Amylase (sweet potato), glucoamylase (Aspergillus niger), and pullulanase (Bacillus acidopullulyticus) from their crude preparations were purified 37-, 31-, and 49-fold with 86, 87, and 95% activity recovery, respectively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed single band in each case.

Entities: Chemical

Mesh：

Substances：

Year: 2002 PMID： 11936253 DOI： 10.1385/MB:20:3:231

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.860

14 in total

6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors: M M Bradford
Journal: Anal Biochem Date: 1976-05-07 Impact factor: 3.365

7. Immobilization of Aspergillus niger xylanase on magnetic latex beads.

Authors: R Tyagi; M N Gupta
Journal: Biotechnol Appl Biochem Date: 1995-04 Impact factor: 2.431

Review 8. Microbial amylolytic enzymes.

Authors: M Vihinen; P Mäntsälä
Journal: Crit Rev Biochem Mol Biol Date: 1989 Impact factor: 8.250

Magnetite-alginate beads for purification of some starch degrading enzymes.

Review 1. Use of magnetic techniques for the isolation of cells.

Review 2. Multistage magnetic and electrophoretic extraction of cells, particles and macromolecules.

3. One step purification of peanut phospholipase D by precipitation with alginate.

4. Purification of alpha-amylases using magnetic alginate beads.

5. The use of paramagnetic beads for the detection of major histocompatibility complex class I and class II antigens.

6. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

7. Immobilization of Aspergillus niger xylanase on magnetic latex beads.

Review 8. Microbial amylolytic enzymes.

Review 9. Alginate as immobilization matrix for cells.

10. Dried calcium alginate/magnetite spheres: A new support for chromatographic separations and enzyme immobilization.

1. Magnetic techniques for the isolation and purification of proteins and peptides.