Y Liu1, L Song, B Li. 1. Department of Pathophysiology, Second Military Medical University, Shanghai 200433, China.
Abstract
OBJECTIVE: To study the molecular mechanism contributing to hormone resistance of nephrotic syndrome. METHODS: The specific hormone binding sites of receptor was determined by radioligand binding assay. Messenger RNA of receptor was determined by quantitative reverse transcription-polymerase chain reaction. RESULTS: No significant differences of specific hormone binding sites of peripheral white blood cells were observed among hormone-sensitive patients, hormone-resistant patients and healthy volunteers. However, in hormone-resistant patients, GR alpha/GR beta mRNA ratio decreased significantly as compared with that of hormone-sensitive patients and healthy volunteers. CONCLUSION: Results of the experiments indicated that the increased expression of GR beta, which leads to the decrease of the GR alpha/GR beta ratio, may be one of the mechanism responsible for hormone resistance in some nephrotic syndrome patients.
OBJECTIVE: To study the molecular mechanism contributing to hormone resistance of nephrotic syndrome. METHODS: The specific hormone binding sites of receptor was determined by radioligand binding assay. Messenger RNA of receptor was determined by quantitative reverse transcription-polymerase chain reaction. RESULTS: No significant differences of specific hormone binding sites of peripheral white blood cells were observed among hormone-sensitive patients, hormone-resistant patients and healthy volunteers. However, in hormone-resistant patients, GR alpha/GR beta mRNA ratio decreased significantly as compared with that of hormone-sensitive patients and healthy volunteers. CONCLUSION: Results of the experiments indicated that the increased expression of GR beta, which leads to the decrease of the GR alpha/GR beta ratio, may be one of the mechanism responsible for hormone resistance in some nephrotic syndromepatients.
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