| Literature DB >> 11927553 |
Toru Tanaka1, Fumihito Hosoi, Yuko Yamaguchi-Iwai, Hajime Nakamura, Hiroshi Masutani, Shugo Ueda, Akira Nishiyama, Shunichi Takeda, Hiromi Wada, Giannis Spyrou, Junji Yodoi.
Abstract
Thioredoxin-2 (Trx-2) is a mitochondria-specific member of the thioredoxin superfamily. Mitochondria have a crucial role in the signal transduction for apoptosis. To investigate the biological significance of Trx-2, we cloned chicken TRX-2 cDNA and generated clones of the conditional Trx-2-deficient cells using chicken B-cell line, DT40. Here we show that TRX-2 is an essential gene and that Trx-2-deficient cells undergo apoptosis upon repression of the TRX-2 transgene, showing an accumulation of intracellular reactive oxygen species (ROS). Cytochrome c is released from mitochondria, while caspase-9 and caspase-3, but not caspase-8, are activated upon inhibition of the TRX-2 transgene. In addition, Trx-2 and cytochrome c are co-immunoprecipitated in an in vitro assay. These results suggest that mitochondrial Trx-2 is essential for cell viability, playing a crucial role in the scavenging ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway.Entities:
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Year: 2002 PMID： 11927553 PMCID： PMC125951 DOI： 10.1093/emboj/21.7.1695
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598