Yong Li1, Lin Yang, Jian-Tao Cui, Wen-Mei Li, Rui-Fang Guo, You-Yong Lu. 1. Beijing Institute for Cancer Research, Beijing Laboratory of Molecular Oncology, School of Oncology, Peking University, 1 Da-Hong-Luo-Chang Street, Western District, Beijing 100034, China.
Abstract
AIM: To elucidate molecular mechanism of chemopreventive efficacies of garlic against human gastric cancer (HGC). METHODS: HGC cell line BGC823 was treated with Allitridi (a kind of garlic extract) and Allitridi-treated and parental BGC823 cDNA libraries were constructed respectively by using lambdaZAP II vector. cDNA Representational Difference Analysis (cDNA RDA) was performed using Bam H I cutting-site and abundant cDNA messages provided by the libraries. Northern blot analysis was applied to identify the obtained difference products. RESULTS: Two specific cDNA fragments were obtained and characterized to be derived from homo sapiens folate receptor alpha (FRalpha) gene and calcyclin gene respectively. Northern blot results showed a 4-fold increase in FRalpha gene expression level and 9-fold increase in calcyclin mRNA level in BGC823 cells after Allitridi treatment for 72h. CONCLUSION: The method of cDNA RDA based on cDNA libraries combines the high specificity of cDNA RDA with abundant cDNA messages in cDNA library; this expands the application of cDNA library and increases the specificity of cDNA RDA. Up-regulation of FRalpha gene and calcyclin gene expressions induced by Allitridi provide valuable molecular evidence for the efficacy garlic in treating HGC as well as other diseases.
AIM: To elucidate molecular mechanism of chemopreventive efficacies of garlic against humangastric cancer (HGC). METHODS: HGC cell line BGC823 was treated with Allitridi (a kind of garlic extract) and Allitridi-treated and parental BGC823 cDNA libraries were constructed respectively by using lambdaZAP II vector. cDNA Representational Difference Analysis (cDNA RDA) was performed using Bam H I cutting-site and abundant cDNA messages provided by the libraries. Northern blot analysis was applied to identify the obtained difference products. RESULTS: Two specific cDNA fragments were obtained and characterized to be derived from homo sapiensfolate receptor alpha (FRalpha) gene and calcyclin gene respectively. Northern blot results showed a 4-fold increase in FRalpha gene expression level and 9-fold increase in calcyclin mRNA level in BGC823 cells after Allitridi treatment for 72h. CONCLUSION: The method of cDNA RDA based on cDNA libraries combines the high specificity of cDNA RDA with abundant cDNA messages in cDNA library; this expands the application of cDNA library and increases the specificity of cDNA RDA. Up-regulation of FRalpha gene and calcyclin gene expressions induced by Allitridi provide valuable molecular evidence for the efficacy garlic in treating HGC as well as other diseases.
Authors: S Miehlke; C Kirsch; B Dragosics; M Gschwantler; G Oberhuber; D Antos; P Dite; J Läuter; J Labenz; A Leodolter; P Malfertheiner; A Neubauer; G Ehninger; M Stolte; E Bayerdörffer Journal: World J Gastroenterol Date: 2001-04 Impact factor: 5.742
Authors: T A Sellers; L H Kushi; J R Cerhan; R A Vierkant; S M Gapstur; C M Vachon; J E Olson; T M Therneau; A R Folsom Journal: Epidemiology Date: 2001-07 Impact factor: 4.822
Authors: W C You; L Zhang; M H Gail; J Y Li; Y S Chang; W J Blot; C L Zhao; W D Liu; H Q Li; J L Ma; Y R Hu; J C Bravo; P Correa; G W Xu; J F Fraumeni Journal: Int J Epidemiol Date: 1998-12 Impact factor: 7.196